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Key Documents

AB1538

Sigma-Aldrich

Anti-Dopamine β Hydroxylase Antibody, NT

Chemicon®, from rabbit

Synonym(s):

DBH

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human, bovine, sheep

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_000787.3

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Anti-Dopamine β Hydroxylase Antibody, NT Chemicon®, from rabbit

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Anti-Dopamine β Hydroxylase Antibody, NT

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Anti-Dopamine β Hydroxylase Antibody

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Anti-DBT antibody produced in rabbit Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab2

HPA026485

Anti-DBT antibody produced in rabbit

biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

rabbit

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

antibody form

affinity isolated antibody

antibody form

serum

antibody form

ascites fluid

antibody form

affinity isolated antibody

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, radioimmunoassay: suitable, western blot: suitable

technique(s)

immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:500-1:1000

clone

polyclonal

clone

polyclonal

clone

4F10.2, monoclonal

clone

polyclonal

Gene Information

human ... DBH(1621)

Gene Information

human ... DBH(1621)

Gene Information

bovine ... Dbh(280758)
rat ... Dbh(25699)

Gene Information

human ... DBT(1629)

Specificity

Dopamine Beta Hydroxylase (DbetaH) N-terminal. By immunoblot the antibody specifically reacts with a single (or double) band(s) of M.W. »70-75,000 in samples of SDS-solubilized, DTT-reduced human adrenal medulla subjected to SDS-PAGE.

Immunogen

Epitope: N-terminus
Synthetic peptide, CSAPRESPLPYHIPLDPEG-amide, cloned from human (DbetaH) cDNA (Nagatsu et al. 1990).

Application

Detect Dopamine β Hydroxylase using this Anti-Dopamine β Hydroxylase Antibody, N-terminus validated for use in IH & WB.
Immunoblotting: 1:250-1:1,000.

Immunohistochemistry: 1:250-1:1,000, staining was evaluated in the locus coeruleus of formaldehyde-fixed, cryopreserved human and monkey brain Showed weak staining on both human and monkey.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

Physical form

Liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Storage and Stability

Maintain at -20 to -70°C in undiluted aliquots for up to six months. Avoid repeated freeze-thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

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