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AB1555

Sigma-Aldrich

Anti-NMDAR2A Antibody

serum, Chemicon®

Synonym(s):

Anti-EPND, Anti-GluN2A, Anti-LKS, Anti-NMDAR2A, Anti-NR2A

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

fish, human, rat, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

suitability

not suitable for immunohistochemistry

NCBI accession no.

NM_000833.2

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biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

species reactivity

human, mouse

species reactivity

mouse, human

species reactivity

human, mouse

species reactivity

mouse

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

antibody form

serum

antibody form

serum

antibody form

purified antibody

antibody form

serum

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

technique(s)

western blot: suitable

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

Specificity

NMDAR2A. By Western blot it recognizes a 180 kDa band in rat brain membranes.

Immunogen

C-Terminal Fusion Protein of the NMDAR2A (30 kDa), aa 1253-1391.

Application

Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
This Anti-NMDAR2A Antibody is validated for use in IP, WB for the detection of NMDAR2A.
Western blotting*: 1:1,000-1:5,000 for ECL. If using labled protein-A for detection: 1:200.

* See suggested protocol

Immunoprecipitation using solubilized hippocampal slices. 3μL will (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2A in 200μg rat brain.

Not suggested for use in immunohistochemistry.

Optimal working dilutions must be determined by the end user.

Physical form

Rabbit antiserum. Liquid, no preservatives.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to one year. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
NMDAR2A is present in high concentrations in the hippocampus

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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    An antisense construct reduces N-methyl-D-aspartate receptor 2A expression and receptor-mediated excitotoxicity as determined by a novel flow cytometric approach.
    Pierre A Mattar, Kevin D Holmes, Gregory A Dekaban
    Journal of Neuroscience Research null
    Differential expression of NMDA and AMPA receptor subunits in rat dorsal and ventral hippocampus.
    C Pandis, E Sotiriou, E Kouvaras, E Asprodini, C Papatheodoropoulos, F Angelatou
    Neuroscience null
    Brain-derived neurotrophic factor and basic fibroblast growth factor downregulate NMDA receptor function in cerebellar granule cells.
    C Brandoli, A Sanna, M A De Bernardi, P Follesa, G Brooker, I Mocchetti
    The Journal of Neuroscience null
    Effects of repeated prenatal glucocorticoid exposure on long-term potentiation in the juvenile guinea-pig hippocampus.
    Setiawan, E; Jackson, MF; MacDonald, JF; Matthews, SG
    The Journal of Physiology null
    Y Honse et al.
    Neuroscience, 122(3), 689-698 (2003-11-19)
    N-methyl-D-aspartate receptor dysfunction has been strongly suggested to link with the abnormalities seen in fetal alcohol syndrome. Thus, the effects of prenatal ethanol exposure on the total expression of NR1 splice variants and the cell surface expression of both NR1
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