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AB3127

Sigma-Aldrich

Anti-FLT-4 Antibody

Chemicon®, from rabbit

Synonym(s):

VEGF Receptor-3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

NCBI accession no.

NM_002020.3
NM_182925.3

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Fenthion

grade

certified reference material, TraceCERT®

grade

certified reference material, TraceCERT®

grade

certified reference material, TraceCERT®

grade

certified reference material, TraceCERT®

product line

TraceCERT®

product line

TraceCERT®

product line

TraceCERT®

product line

TraceCERT®

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

manufacturer/tradename

Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

manufacturer/tradename

Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

manufacturer/tradename

Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

300

Specificity

Recognizes mouse Flt-4 (mouse 1363-aa and human 1298-aa) a receptor protein tyrosine kinase. It is type 1 membrane protein, a receptor for VEGF-C and is expressed in adult lung, and liver, and in fetal liver, brain, intestine, and placenta. Flt-4 is also known as vascular endothelial growth factor receptor 3 (VEGFR3).

SPECIES REACTIVITIES: The peptide sequence used is relatively conserved in rat (85%) and human (73%) Flt-4/VEGFR3. No significant sequence homology is seen with VEGFR1, VEGFR2, or other tyrosine kinases. Antibody crossreactivity in other species is not established.

Immunogen

20 amino acid peptide sequence within the cytoplasmic, C-terminus of mouse Flt-4

Application

Detect FLT-4 using this Anti-FLT-4 Antibody validated for use in ELISA & WB.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors
Western blot: 1-10 μg/mL using ECL. The antibodies detected ~140 kDa band in rat liver extracts.

ELISA: 1:10,000-1:100,000, using 50-100ng of control peptide/well.

Optimal working dilutions must be determined by end user.

Physical form

Liquid in PBS, containing 0.1% BSA. Affinity purified immunoglobulin.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze and thaw.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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O Aprelikova et al.
Cancer research, 52(3), 746-748 (1992-02-01)
The receptors for at least two hematopoietic growth factors, namely the stem cell factor and colony-stimulating factor 1, belong to class III receptor tyrosine kinases. Here we describe cloning of a partial complementary DNA for FLT4, an additional member of
H Finnerty et al.
Oncogene, 8(8), 2293-2298 (1993-08-01)
A wide range of growth and differentiation processes are regulated by the signalling of receptor tyrosine kinases (RTKs). We have developed a nested polymerase chain reaction (PCR) procedure with degenerate primers, and used it to identify RTKs expressed in murine

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