AB3609
Anti-Smac/Diablo Antibody
Chemicon®, from rabbit
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... DIABLO(56616)
General description
The inhibitor of apoptosis (IAP) proteins regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes their inhibitory effect on caspases has been designated Smac/DIABLO. This is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates the cytochrome c/Apaf-1/caspase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.
Specificity
Detects Smac/DIABLO in an approximately 25 kDa band by western blot.
Immunogen
Peptide corresponding to aa 225-239 (EERAESEQEAYLRED) of human Smac/DIABLO.
Application
This Anti-Smac/Diablo Antibody is validated for use in WB for the detection of Smac/Diablo.
Western blot: 1:500 to 1:1000
Human heart tissue lysate can be used as a positive control.
Optimal working dilutions must be determined by end user.
Human heart tissue lysate can be used as a positive control.
Optimal working dilutions must be determined by end user.
Physical form
Format: Purified
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
G Wu et al.
Nature, 408(6815), 1008-1012 (2001-01-05)
Apoptosis is an essential process in the development and homeostasis of all metazoans. The inhibitor-of-apoptosis (IAP) proteins suppress cell death by inhibiting the activity of caspases; this inhibition is performed by the zinc-binding BIR domains of the IAP proteins. The
Long lasting heat shock stimulation of TRAIL-induced apoptosis in transformed T lymphocytes.
Maryline Moulin, Andre-Patrick Arrigo
Experimental Cell Research null
Z Liu et al.
Nature, 408(6815), 1004-1008 (2001-01-05)
The inhibitor-of-apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. Recently, a mammalian protein called Smac (also named DIABLO) was identified that binds to the IAPs and promotes caspase activation. Although undefined in
APOLLON protein promotes early mitotic CYCLIN A degradation independent of the spindle assembly checkpoint.
Kikuchi, R; Ohata, H; Ohoka, N; Kawabata, A; Naito, M
The Journal of Biological Chemistry null
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Malkesman, O; Austin, DR; Tragon, T; Henter, ID; Reed, JC; Pellecchia, M; Chen, G; Manji, HK
Molecular Psychiatry null
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