AB5320
Anti-CSPG4 Antibody
CHEMICON®, rabbit polyclonal
Synonym(s):
Chondroitin sulfate proteoglycan NG2, Melanoma chondroitin sulfate proteoglycan 3, Melanoma-associated chondroitin sulfate proteoglycan, chondroitin sulfate proteoglycan 4, chondroitin sulfate proteoglycan 4 (melanoma-associated)
About This Item
Product Name
Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, Chemicon®, from rabbit
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse, monkey, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
1 of 4
This Item | Y0001209 | PHR1324 | 1.06143 |
---|---|---|---|
grade pharmaceutical primary standard | grade pharmaceutical primary standard | grade certified reference material, pharmaceutical secondary standard | grade - |
manufacturer/tradename USP | manufacturer/tradename EDQM | manufacturer/tradename - | manufacturer/tradename - |
application(s) pharmaceutical (small molecule) | application(s) pharmaceutical (small molecule) | application(s) pharmaceutical (small molecule) | application(s) liquid formulation |
format neat | format neat | format neat | format - |
API family meglumine | API family meglumine | API family meglumine | API family - |
autoignition temp. ~662 °F | autoignition temp. ~662 °F | autoignition temp. ~662 °F | autoignition temp. ~662 °F |
General description
Specificity
Immunogen
Application
A 1:150-1:600 dilution of a previous lot was used in IC.
Immunohistochemistry:
1:200 dilution of a previous lot was used on frozen sections of embryonic mouse brain using an Alexa Fluor conjugated secondary antibody. Care should be taken to avoid overfixing tissue sections. A short (5-10 minute) fix in 2-4% PFA is recommended.
Immunoprecipitation:
2 μg/mL concentration of a previous lot was used in IP.
ELISA:
A 1:1,500-1:3,000 dilution of a previous lot was used in ELISA.
Optimal working dilutions and protocols must be determined by end user
Neuroscience
Neuronal & Glial Markers
Quality
Western Blot: 1:1000 dilution of this lot detected NG2 chondroitin sulfate on 10 μg of rat brain lysates.
Target description
Physical form
Storage and Stability
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Human melanoma, glioma and proliferating brain endothelial cells, rat B49 glial cell extracts or embryonic mouse brain tissue.
Other Notes
Legal Information
Disclaimer
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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