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Key Documents

ABE570

Sigma-Aldrich

Anti-RINF (CXXC5)

from rabbit, purified by affinity chromatography

Synonym(s):

CXXC-type zinc finger protein 5, CF5, Putative MAPK-activating protein PM08, Putative NF-kappa-B-activating protein 102, Retinoid-inducible nuclear factor, RINF

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

rat (based on 100% sequence homology), mouse (based on 100% sequence homology)

packaging

antibody small pack of 25 μg

technique(s)

western blot: suitable

General description

CXXC-type zinc finger protein 5 (UniProt: Q7LFL8; also known as CF5, Putative MAPK-activating protein PM08, Putative NF-kappa-B-activating protein 102, Retinoid-inducible nuclear factor, RINF) is encoded by the CXXCS (also known as HSPC195, TCCCIA00297) gene (Gene ID: 51523) in human. In human cells, two isoforms of RINF have been reported that are produced by alternative splicing. The isoform two lacks the first 95 amino acids. RINF serves as a transcription factor that binds to the oxygen responsive element of COX4I2 and represses its transcription under hypoxia conditions (4% oxygen), as well as normoxia conditions (20% oxygen). Its nuclear localization sequence is localized in amino acids 257-262. It is shown to co-localize with DVL1 in large bodies localized outside the nuclear membrane. RINF is shown to be important in normal myelopoiesis. RINF acts as a mediator of BMP4-mediated modulation of canonical Wnt signaling activity in neural stem cells. It is also shown to be essential for DNA damage-induced ATM phosphorylation, p53 activation, and cell cycle arrest. RINF silencing is reported to increase sensitivity of K562 cells to daunorubicin- and lenalidomide. (Ref.: Aston A et al. (2013). Oncotarget. 4(9); 1438-1448).

Specificity

This rabbit polyclonal antibody detects CXXC-type zinc finger protein 5 (RINF) in human cells. It targets an epitope within 17 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of human CXXC-type zinc finger protein 5. Immunogen is conserved between two isoforms.

Application

Anti-RINF (CXXC5) Antibody, Cat. No. ABE570, is a highly specific rabbit polyclonal antibody that targets RINF (CXXC5) and has been tested in Western Blotting.

Quality

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected RINF (CXXC5) in 10 µg of MCF-7 cell lysate.

Target description

~35 kDa observed; 32.98 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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