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ABN481

Sigma-Aldrich

Anti-Synaptopodin Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Synaptopodin, SYNPO

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.
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biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human

technique(s)

immunohistochemistry: suitable
western blot: suitable

UniProt accession no.

shipped in

wet ice

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SAB3500585ABT335SAB1402860
Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

mouse

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

indirect ELISA: suitable, western blot: 1-5 μg/mL

species reactivity

rat, human

species reactivity

mouse, rat, human

species reactivity

human, mouse

species reactivity

human

General description

Synaptopodin (SYNPO) is an actin-associated protein that may play an important role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. It is expressed in the cerebral cortex. Mutations in SYNPO may be associated with nephrotic syndrome.

Immunogen

Epitope: Near C-terminus.
KLH-conjugated linear peptide corresponding to a sequence near the C-terminus of human SYNPO.

Application

Detect Synaptopodin using this rabbit polyclonal antibody, Anti-Synaptopodin Antibody validated for use in western blotting & IHC.
Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected SYNPO in human brain tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected SYNPO in 15 µg of rat brain tissue lysate.

Target description

~95 kDa observed. Uniprot describes three isoforms at ~74 kDa, ~96 kDa, and ~97 kDa Uncharacterized bands may appear in some lysates.

Physical form

Affinity purified.
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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