ABS2123
Anti-PPOX
from rabbit
Synonym(s):
Protoporphyrinogen oxidase, PPOX
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
unpurified
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse
species reactivity (predicted by homology)
human (based on 100% sequence homology)
packaging
antibody small pack of 25 μL
technique(s)
western blot: suitable
isotype
IgG
General description
Protoporphyrinogen oxidase (UniProt: P50336; also known as EC: 1.3.3.4, PPO, PPOX) is encoded by the PPOX gene (Gene ID: 5498) in human. Protoporphyrinogen oxidase is a mitochondrial inner membrane enzyme that catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX. It is involved in step one of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX. It is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas and may be present as a monomer or active homodimeric form. Defects in PPOX are linked to variegate porphyria that lead to severe deficiency of this enzyme that result in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver.
Specificity
This rabbit polyclonal antibody detects Protoporphyrinogen oxidase (PPO) in Human and Mouse cells.
Immunogen
Epitope: unknown
Full length recombinant human Protoporphyrinogen oxidase (PPOX).
Application
Anti-PPOX, Cat. No. ABS2123, is a rabbit polyclonal antibody that detects Protoporphyrinogen oxidase and has been tested for use in Western Blotting.
Research Category
Signaling
Signaling
Western Blotting Analysis: A representative lot detected PPOX in undifferentiated (day 0) and maturing (day 3 or 4) mouse erythroleukemia (MEL) cells (Chung, J., et. al. (2015). Sci Signal. 8(372):ra34). and differentiated Mouse erythroleukemia (MEL) cells (Medlock, A.E., et. al. (2015). PLoS One. 10(8):e0135896).
Quality
Evaluated by Western Blotting in lystate from mitochondria isolated from differentiated DS19 cells.
Western Blotting Analysis: A 1:500 dilution of this antibody detected PPOX in lystate from mitochondria isolated from differentiated DS19 cells.
Western Blotting Analysis: A 1:500 dilution of this antibody detected PPOX in lystate from mitochondria isolated from differentiated DS19 cells.
Target description
~53 kDa observed; 50.77 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Unpurified
Rabbit polyclonal antiserum with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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