CB1044
Anti-OCLN Mouse mAb (1G7)
liquid, clone 1G7, Calbiochem®
Synonym(s):
Anti-Occludin
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
1G7, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
General description
Purified mouse monoclonal antibody. Recognizes the ~55-60 kDa OCLN protein.
Recognizes the ~55-60 kDa OCLN protein in HL-60 and HeLa cells.
This Anti-OCLN Mouse mAb (1G7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of OCLN.
Immunogen
A recombinant polypeptide corresponding to amino acids of 423-523 human OCLN, expressed as a GST fusion protein
Application
ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Warning
Toxicity: Regulatory Review (Z)
Physical form
In PBS, pH 7.2.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HL-60 cells, HeLa cells
HL-60 cells, HeLa cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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