HMHEMAG-34K
MILLIPLEX® Human Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay
The Human Metabolic Hormone Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 13 protein biomarkers in human serum, plasma and cell/tissue culture samples.
Synonym(s):
human metabolic hormone immunoassay panel, luminex human metabolic hormone multiplex assay, millipore human metabolism multiplex kit
About This Item
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species reactivity
human
manufacturer/tradename
Milliplex®
assay range
accuracy: 101%
(Glucagon)
accuracy: 102%
(Leptin)
accuracy: 103%
(GIP)
accuracy: 103%
(GLP-1 (Total))
accuracy: 105%
(Ghrelin (Active))
accuracy: 106%
(MCP-1)
accuracy: 97%
(TNFα)
accuracy: 99%
(C-Peptide)
sensitivity: 0.5 pg/mL
(MinDC+2SD; TNF?)
sensitivity: 1.1 pg/mL
(MinDC+2SD; GIP)
sensitivity: 13 pg/mL
(MinDC+2SD; IL-6)
sensitivity: 14 pg/mL
(MinDC+2SD; Ghrelin (Active))
sensitivity: 14 pg/mL
(MinDC+2SD; MCP-1)
sensitivity: 146 pg/mL
(MinDC+2SD; PYY)
sensitivity: 15 pg/mL
(MinDC+2SD; Amylin (Total))
sensitivity: 16 pg/mL
(MinDC+2SD; C-Peptide)
sensitivity: 16 pg/mL
(MinDC+2SD; Glucagon)
sensitivity: 160 pg/mL
(MinDC+2SD; Insulin)
sensitivity: 2.4 pg/mL
(MinDC+2SD; GLP-1 (Active))
sensitivity: 2.9 pg/mL
(MinDC+2SD; PP)
sensitivity: 28 pg/mL
(MinDC+2SD; Amylin (Active))
sensitivity: 64 pg/mL
(MinDC+2SD; Leptin)
sensitivity: 7.3 pg/mL
(MinDC+2SD; GLP-1 (Total))
standard curve range: 0.69-500 pg/mL
(TNFα)
standard curve range: 1.4-1,000 pg/mL
(GIP)
standard curve range: 13.7-10,000 pg/mL
(Amylin (Active))
standard curve range: 13.7-10,000 pg/mL
(Amylin (Total))
standard curve range: 13.7-10,000 pg/mL
(Ghrelin (Active))
standard curve range: 13.7-10,000 pg/mL
(Glucagon)
standard curve range: 13.7-10,000 pg/mL
(IL-6)
standard curve range: 13.7-10,000 pg/mL
(MCP-1)
standard curve range: 13.7-10,000 pg/mL
(PYY)
standard curve range: 137-100,000 pg/mL
(Insulin)
standard curve range: 137-100,000 pg/mL
(Leptin)
standard curve range: 2.7-2,000 pg/mL
(GLP-1 (Active))
standard curve range: 2.7-2,000 pg/mL
(GLP-1 (Total))
standard curve range: 2.7-2,000 pg/mL
(PP)
standard curve range: 27.4-20,000 pg/mL
(C-Peptide)
inter-assay cv: <15%
intra-assay cv: <10%
(C-Peptide)
inter-assay cv: <15%
intra-assay cv: <10%
(GIP)
inter-assay cv: <15%
intra-assay cv: <10%
(GLP-1 (Active))
inter-assay cv: <15%
intra-assay cv: <10%
(GLP-1 (Total))
inter-assay cv: <15%
intra-assay cv: <10%
(Ghrelin (Active))
inter-assay cv: <15%
intra-assay cv: <10%
(Glucagon)
inter-assay cv: <15%
intra-assay cv: <10%
(IL-6)
inter-assay cv: <15%
intra-assay cv: <10%
(Insulin)
inter-assay cv: <15%
intra-assay cv: <10%
(Leptin)
inter-assay cv: <15%
intra-assay cv: <10%
(MCP-1)
inter-assay cv: <15%
intra-assay cv: <10%
(PP)
inter-assay cv: <15%
intra-assay cv: <10%
(PYY)
inter-assay cv: <15%
intra-assay cv: <10%
(TNFα)
inter-assay cv: <20%
intra-assay cv: <10%
(Amylin (Active))
inter-assay cv: <20%
intra-assay cv: <10%
(Amylin (Total))
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
wet ice
1 of 4
This Item | SML1655 | SML1728 | SML1521 |
---|---|---|---|
form powder | form powder | form powder | form powder |
assay ≥95% (HPLC) | assay ≥97% (HPLC) | assay ≥98% (HPLC) | assay ≥98% (HPLC) |
Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
storage temp. room temp | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. room temp |
storage condition protect from light | storage condition - | storage condition protect from light | storage condition - |
solubility DMSO: 15 mg/mL, clear | solubility DMSO: 2 mg/mL, clear (warmed) | solubility DMSO: 10 mg/mL, clear (warmed) | solubility DMSO: 15 mg/mL, clear |
General description
The MILLIPLEX® Human Metabolic Hormone Panel is a 15 analyte, 13-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and cell/tissue culture samples: Amylin (Active) or Amylin (Total), C-Peptide, Ghrelin (Active), GIP, GLP-1 (Active) or GLP-1 (Total), Glucagon, IL-6, Insulin, Leptin, MCP-1, PP, PYY, TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Metabolism
Application
- Analytes: Amylin (Active), Amylin (Total), C-Peptide, Ghrelin (Active), GIP (Total), GLP-1 (Active), GLP-1 (Total), Glucagon, IL-6, Insulin, Leptin, MCP-1 (CCL2), Pancreatic Polypeptide (PP), PYY (Total), TNF-α
- NOTE: The following analytes cannot be assayed simultaneously: Amylin (Active) & Amylin (Total); GLP-1 (Active) & GLP-1 (Total).
- Recommended Sample Type: Human serum, plasma, and cell/tissue culture supernatants or lysates
- Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma; cell/tissue culture samples may be used undiluted or diluted with an appropriate control medium NOTE: The following analytes require the addition of protease inhibitors to serum or plasma samples (see sample collection and storage section in the protocol for details): GLP-1 (DPPIV inhibitor); Amylin (protease inhibitor cocktail); Glucagon (Aprotinin); Ghrelin (Active) (serine protease inhibitor).
- Assay Run Time: Overnight (16-18 hours) at 2-8°C
- Research Category: Metabolism
- Research Subcategory: Metabolic Disorders
Features and Benefits
Linkage
Other Notes
Legal Information
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Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
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