MAB1961H-I

Anti-Integrin αVβ5 Antibody, clone P1F6, Phycoerythrin conjugate

clone P1F6, from mouse, PE

• Synonym(s): Integrin alpha-V, CD51, Integrin alpha V, Vitronectin receptor subunit alpha, Integrin beta-5
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: PE
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: P1F6, monoclonal
• species reactivity: human, hamster, chicken
• technique(s): flow cytometry: suitable
• isotype: IgG1
• NCBI accession no.: NM_002210.4; NM_002213.4

Description

General description

Integrin alpha-V (UniProt P06756; CD51, Integrin alpha V, Vitronectin receptor subunit alpha) and Integrin beta-5 (UniProt P18084) are encoded by the ITGAV (MSK8, VNRA, VTNR; Gene ID 3685) and the ITGB5 (Gene ID 3693) gene, respectively, in human. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and a beta subunit associated in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence present in the extracellular matrix components, such as fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. Integrin alphaVbeta5 is the primary mechanotransducer that mediates lung myofibroblast contraction-induced latent TGF-beta1 activation. Increased alphaVbeta5 expression is reported to be associated with myofibroblast differentiation. Integrin alphaVbeta5 is shown to interact with Thy-1 in an RLD-dependent manner. Thy-1 expression prevents fibroblast contraction-induced latent TGF-beta1 activation and lung myofibroblast differentiation.

Specificity

Clone P1F6 recognizes αVβ5 without cross-reactivity toward αVβ3 (Weinacker, A., et al. (1994). J. Biol. Chem. 269(9):6940-6948).

Immunogen

Epitope: Extracellular region.

Human UCLA P3 lung carcinoma cells (Weinacker, A., et al. (1994). J. Biol. Chem. 269(9):6940-6948).

Application

Anti-Integrin αVβ5 Antibody, clone P1F6, Phycoerythrin conjugate is an antibody against Integrin αVβ5 for use in Flow Cytometry.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

The unconjugated antibody (Cat. No. MAB1961 & MAB1961Z) is shown to be suitable also for Flow cytometry, Functional blocking, Immunocytochemistry, Immunohistochemistry, and Immunoprecipitation applications.

Quality

Evaluated by Flow Cytometry in HT1080 cells.

Flow Cytometry Analysis: 1.0 µg of this antibody detected Integrin αVβ5 in HT1080 cells.

Target description

112.7 kDa (alpha-V) and 86.34 kDa (beta-5) calculated. ~150 kDa (alpha-V) and 95 kDa (beta-5) reported (Weinacker, A., et al. (1994). J. Biol. Chem. 269(9):6940-6948) due to glycosylation.

Physical form

Protein A purified

Purified mouse monoclonal IgG1 antibody in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable