Skip to Content
MilliporeSigma
All Photos(2)

Key Documents

MAB2252

Sigma-Aldrich

Anti-Integrin β1 Antibody, clone N29

clone N29, Chemicon®, from mouse

Synonym(s):

CD29, MAB2252Z

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Pricing and availability is not currently available.

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

N29, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

cell culture | mammalian: suitable
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgGκ

NCBI accession no.

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
N5037S9684C2724
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

mol wt

antigen 48 kDa

mol wt

antigen 140 kDa

mol wt

antigen 25 kDa

mol wt

antigen 28 kDa

species reactivity

mouse, rat

species reactivity

rat, mouse, canine

species reactivity

rat, mouse

species reactivity

rat

Specificity

Reacts with the human integrin beta1 subunit. Specificity verified by preclearing of beta1in immunoprecipitation, flow cytometry on transfectant cells displaying human beta1 integrin, and reactivity with purified beta1. Recognizes an epitope cluster distinct from MAB2250 and MAB2251(Wilkins et al., 1996).

Immunogen

Jurkat T-leukemic cell line

Application

Immunohistochemistry (Paraffin) Analysis: A 1:50-2,000 dilution from a representative lot detected Integrin β1 in the membrane of tubule epithelial cells and cells of the glomeruli in human kidney tissue, membrane of alveolar cells, endothelial cells, and bronchiole epithelial cells of human lung tissue.

Immunoprecipitation: A representative lot of this antibody clone was used in immunoprecipitation.

Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry on frozen sections.

Flow Cytometry: A representative lot of this antibody clone was used in flow cytometry.

Functional Activity Assay: A representative lot of this antibody clone was used to stimulate adhesion of cells to extracellular matrix proteins (Wilkins, J.A. et al., 1996).

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins
This Anti-Integrin β1 Antibody, clone N29 is validated for use in FC, IH, IP, FUNC, CULT, WB for the detection of Integrin β1.

Quality

Western Blot Analysis: 0.5 µg/mL of the antibody detected Integrin β1 in 10 µg of U251 cell lysate.

Target description

88 kDa

Physical form

Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Tonsil, human skin, human kidney tissue

U251 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


  • Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

    Need A Sample COA?

    This is a sample Certificate of Analysis (COA) and may not represent a recently manufactured lot of this specific product.

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Competitive binding of Rab21 and p120RasGAP to integrins regulates receptor traffic and migration.
    Mai, A; Veltel, S; Pellinen, T; Padzik, A; Coffey, E; Marjomaki, V; Ivaska, J
    The Journal of cell biology null
    Mammary-derived growth inhibitor alters traffic of EGFR and induces a novel form of cetuximab resistance.
    Jonna Nevo,Elina Mattila,Teijo Pellinen,Daniel L Yamamoto,Henri Sara,Kristiina Iljin et al.
    Clinical cancer research : an official journal of the American Association for Cancer Research null
    Steven Mortillo et al.
    The Journal of comparative neurology, 520(9), 2041-2052 (2012-04-11)
    β1-containing integrins are required for persistent synaptic potentiation in hippocampus and regulate hippocampal-dependent learning. Based largely on indirect evidence, there is a prevailing assumption that β1-integrins are localized at synapses, where they contribute to synapse adhesion and signaling, but this
    Distinct recycling of active and inactive ?1 integrins.
    Arjonen, A; Alanko, J; Veltel, S; Ivaska, J
    Traffic null
    P L Bigliardi et al.
    British journal of pharmacology, 172(2), 501-514 (2014-03-19)
    In addition to its analgesic functions, the peripheral opioid receptor system affects skin homeostasis by influencing cell differentiation, migration and adhesion; also, wound healing is altered in δ-opioid receptor knockout mice (DOPr(-/-) ). Hence, we investigated δ-opioid receptor effects on

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service