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MAB2258

Sigma-Aldrich

Anti-Syntaxin-3 Antibody, clone 1-146

clone 1-146, from mouse

Synonym(s):

syntaxin 3, syntaxin 3A

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

1-146, monoclonal

species reactivity

human, rat

species reactivity (predicted by homology)

mouse

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_112386

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Anti-Syntaxin-3 Antibody, clone 1-146 clone 1-146, from mouse

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Gene Information

human ... STX3(6809)
rat ... Stx3(81802)

Gene Information

mouse ... Stx3(20908)

Gene Information

human ... STX3(6809)

Gene Information

human ... STX1A(6804)

species reactivity

human, rat

species reactivity

mouse, human, bovine

species reactivity

human

species reactivity

hamster, human, pig, rat

antibody form

purified antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

ascites fluid

clone

1-146, monoclonal

clone

12E5, monoclonal

clone

polyclonal

clone

SP8, monoclonal

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

mouse

isotype

IgG1κ

isotype

IgG2aκ

isotype

-

isotype

IgG1

General description

Syntaxin-3, also known as Stx3, is a member of the SNARE family which are complexes involved in membrane fusion. Syntaxin-3 is expressed in epithelial cells and is found apically in MDCK and Caco-2 cells (1).

Specificity

This antibody recognizes Syntaxin-3.

Immunogen

GST-tagged recombinant protein corresponding to residues contained within rat Syntaxin-3.

Application

Detect Syntaxin-3 using this Anti-Syntaxin-3 Antibody, clone 1-146 validated for use in WB, IP, IH.
Research Category
Neuroscience
Research Sub Category
Synapse & Synaptic Biology

Quality

Evaluated by Western Blot in rat brain tissue lysate.
Western Blot Analysis: : 0.5 µg/mL dilution of this antibody detected Syntaxin-3 on 15 µg of rat brain tissue lysate.

Target description

Approx. 33 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl with 0.05% NaN3.

Storage and Stability

Maintain refrigerated at 2-8°C for 1 year from date of receipt.

Analysis Note

Control
Rat brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

    • Certificates of Analysis (COA)

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    Daniëlle Rianne José Verboogen et al.
    eLife, 6 (2017-05-20)
    SNARE proteins play a crucial role in intracellular trafficking by catalyzing membrane fusion, but assigning SNAREs to specific intracellular transport routes is challenging with current techniques. We developed a novel Förster resonance energy transfer-fluorescence lifetime imaging microscopy (FRET-FLIM)-based technique allowing
    Adrian J Giovannone et al.
    Molecular biology of the cell, 28(21), 2843-2853 (2017-08-18)
    Syntaxin 3 (Stx3), a SNARE protein located and functioning at the apical plasma membrane of epithelial cells, is required for epithelial polarity. A fraction of Stx3 is localized to late endosomes/lysosomes, although how it traffics there and its function in
    Hiroshi Gomi et al.
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 65(11), 637-653 (2017-09-16)
    The comparative structure and expression of salivary components and vesicular transport proteins in the canine major salivary glands were investigated. Histochemical analysis revealed that the morphology of the five major salivary glands-parotid, submandibular, polystomatic sublingual, monostomatic sublingual, and zygomatic glands-was
    Poppy Datta et al.
    The Journal of biological chemistry, 294(50), 19119-19136 (2019-11-07)
    Mutations in the centrosomal protein 290 (CEP290) gene cause various ciliopathies involving retinal degeneration. CEP290 proteins localize to the ciliary transition zone and are thought to act as a gatekeeper that controls ciliary protein trafficking. However, precise roles of CEP290
    Poppy Datta et al.
    PloS one, 16(5), e0246358-e0246358 (2021-05-08)
    Nephrocystin (NPHP1) is a ciliary transition zone protein and its ablation causes nephronophthisis (NPHP) with partially penetrant retinal dystrophy. However, the precise requirements of NPHP1 in photoreceptors are not well understood. Here, we characterize retinal degeneration in a mouse model
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