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MAB3083

Sigma-Aldrich

Anti-Calpain Antibody, small subunit of µ- or m-Calpains (Calpain I or II), clone P1

ascites fluid, clone P1, Chemicon®

Synonym(s):

Anti-CALPAIN4, Anti-CANP, Anti-CANPS, Anti-CAPN4, Anti-CDPS, Anti-CSS1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

P1, monoclonal

species reactivity

human, bovine

should not react with

rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

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This Item
SAB1405555AB81023SAB5700145
Anti-Calpain Antibody, small subunit of µ- or m-Calpains (Calpain I or II), clone P1 ascites fluid, clone P1, Chemicon®

MAB3083

Anti-Calpain Antibody, small subunit of µ- or m-Calpains (Calpain I or II), clone P1

Anti-CAPNS1 antibody produced in mouse purified immunoglobulin, buffered aqueous solution

SAB1405555

Anti-CAPNS1 antibody produced in mouse

Anti-Calpain II Antibody, large subunit Chemicon®, from rabbit

AB81023

Anti-Calpain II Antibody, large subunit

Anti-CAPN2 antibody produced in rabbit

SAB5700145

Anti-CAPN2 antibody produced in rabbit

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

rabbit

Gene Information

human ... CAPNS1(826)

Gene Information

human ... CAPNS1(826)

Gene Information

human ... CAPN2(824)

Gene Information

human ... CAPN2(824)

clone

P1, monoclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

affinity isolated antibody

species reactivity

human, bovine

species reactivity

human

species reactivity

mouse, rat, human

species reactivity

human, mouse

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

Calpains are non-lysosomal cysteine proteeases which absolutely require calcium for activity. There are two major calpain isozymes: μ-calpain and m-calpain, which require micromolar or millimolar calicum for activity, respectively, in invitro assays. The major calpain isozymes are composed of two subunits: an 80kDa catalytic subunit; and a 30kDa regulatory subunit. The large and small subunits remain associated in the presence of calcium after proteolytic activation of the zymogen.

Specificity

Reacts with the small subunit of human erthrocyte μ-calpain (Calpain I) or bovine myocardial m-calpain (Calpain II).

Immunogen

Bovine myocardial m-calpain.
Epitope: small subunit of μ- or m-Calpains (Calpain I or II)

Application

Immunoblot: 1:500-1,000. The small subunit of either μ-calpain m-calpain is 30kDa. ELISA: 1:50,000 (against the immunogen). Immunohistochemistry: 1:100 on 4% paraformaldehyde or 1% paraformaldehyde/4% glutaraldehyde fixed tissue. Immunocytochemistry: 1:100. Optimal working dilutions must be determined by the end user.
This Anti-Calpain Antibody, small subunit of μ-or m-Calpains (Calpain I or II), clone P1 is validated for use in ELISA, WB, IC, IH for the detection of Calpain.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J M Harlan et al.
Laboratory investigation; a journal of technical methods and pathology, 48(3), 269-274 (1983-03-01)
Lipopolysaccharide (LPS) produced time- and dose-dependent bovine endothelial cell injury in vitro that was manifested initially by cell detachment from culture substrate with subsequent cell lysis. Bovine endothelial cell injury was observed with LPS derived from Salmonella minnesota R595, a

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