MAB5224-I

Anti-Internexin α Antibody, CT Antibody

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2E3, monoclonal
• mol wt: calculated mol wt 56.12 kDa; observed mol wt ~60 kDa
• species reactivity: feline, rat, human, mouse
• packaging: antibody small pack of 100 μg
• technique(s): ELISA: suitable; electron microscopy: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG1κ
• UniProt accession no.: P23565
• shipped in: dry ice
• storage temp.: -10 to -25°C
• target post-translational modification: unmodified
• Gene Information: rat ... Ina(24503)

Description

General description

Alpha-Internexin (UniProt: P23565; also known as Alpha-Inx) is encoded by the Ina (also known as Inexa) gene (Gene ID: 24503) in rat. Alpha-Internexin is class IV neuronal intermediate filament that self-assemble. It can function as a homodimer to form an independent structural network or may cooperate with NF-L protein to form the filamentous backbone to which Neurofilament medium (NEFM) and neurofilament heavy (NEFH) polypeptides attach to form the cross-bridges. It may also cooperate with the neuronal intermediate filament protein PRPH to form filamentous networks. It plays a role in the morphogenesis of neurons. It is expressed in large amounts early in neuronal development but is downregulated in many neurons as the development progresses. At embryonic day 16, when the expression of neurofilament-light (NF-L) is limited to a relatively small number of neurons and at low expression, alpha-internexin levels are found to be significantly higher. It has also been reported that despite a postnatal decline in expression, alpha-internexin is abundant as the triplet (NF-L, NF-M, and NF-H) in the adult CNS and exists in a relatively fixed stoichiometry with these subunits. Overexpression of alpha-internexin in transgenic mice results in the formation of cerebellar torpedoes. These torpedoes appear as swellings in Purkinje cell axons. Their presence results in the degeneration and ultimate death of the Purkinje cells. (Ref.: Kaplan, MP., et al. (1990). J. Neurosci. 10(8); 2735-2748; Evans, J., et al. (2002). J. Neurophysiol. 87(2); 1076-1085; Yuan, A., et al. (2006). J. Neurosci. 26(39); 10006-10019).

Specificity

Clone 2E3 is a mouse monoclonal antibody that detects Internexin a. It targets an epitope within the C-terminal region.

Immunogen

Full length recombinant rat Internexin a.

Application

Quality Control Testing

Evaluated by Western Blotting in Rat brain stem tissue lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Internexin a in Rat brain stem tissue lysates.

Tested Applications

ELISA Analysis: A representative lot detected Internexin a in ELISA applications (Evans, J., et. al. (2002). J Neurohysiol. 87(2):1076-85).

Immunocytochemistry Analysis: A representative lot detected Internexin a in Immunocytochemistry applications (Yuan, A., et. al. (2006). J Neurosci. 26(39):10006-19; Evans, J., et. al. (2002). J Neurohysiol. 87(2):1076-85).

Electron Microscopy Analysis: A representative lot of this antibody was used for ultrastructural colocalization of Internexin a and NF-M on neurofilament in optic nerve of adult mice. (Yuan, A., et. al. (2006). J Neurosci. 26(39):10006-19).

Western Blotting Analysis: A representative lot detected Internexin a in Western Blotting applications (Yuan, A., et. al. (2006). J Neurosci. 26(39):10006-19; Evans, J., et. al. (2002). J Neurohysiol. 87(2):1076-85 ).

Immunohistochemistry Applications: A representative lot detected Internexin a in Immunohistochemistry applications ( Yuan, A., et. al. (2006). J Neurosci. 26(39):10006-19).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Internexin a, CT, clone 2E3, Cat. No. MAB5224-I, is a mouse monoclonal antibody that detects Internexin a and is tested for use in Electron Microscopy, ELISA, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing PBS with 5 mM sodium azide with 50% glycerol.

Storage and Stability

Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable