MABC954
Anti-MUTYH Antibody, clone 12D5.1
clone 12D5.1, from mouse
Synonym(s):
A/G-specific adenine DNA glycosylase, MutY homolog, hMYH
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
General description
A/G-specific adenine DNA glycosylase (UniProt Q9UIF7; also known as hMYH, MutY homolog) is encoded by the MUTYH (also known as MYH) gene (Gene ID 4595) in human. Base excision repair (BER) is initiated by damage-specific DNA glycosylases that recognize and remove aberrant bases by hydrolyzing the N-glycosidic bond, creating an apurinic/apyrimidinic (AP) site. This is followed by an (AP)-endonuclease-catalyzed DNA strand incision 5′ to the AP site, trimming of the 5′ end by an (AP)-lyase or an endonuclease, DNA resynthesis by a DNA polymerase and strand sealing by a DNA ligase. Monofunctional glycosylases have only glycosylase activity, whereas bifunctional glycosylases possess additional AP lyase activity and can therefore convert a base lesion into a single-strand break without the need for a separate AP endonuclease. MUTYH/MYH is a monofunctional DNA glycosylase that excises mismatched adenine opposite 7, 8-dihydro-8-oxoguanine (8-oxoG), which is the major mutagenic lesion induced by oxidative stress. Biallelic mutations in MUTYH are associated with MUTYH-Associated polyposis (MAP) and increased risk in colorectal cancer (CRC).
Specificity
Expected to react with all six spliced isoforms of human MUTYH reported by UniProt (Q9UIF7).
Immunogen
Epitope: N-terminal half.
GST-tagged recombinant internal fragment from the N-terminal half of human MUTYH.
Application
Anti-MUTYH Antibody, clone 12D5.1 is an antibody against MUTYH for use in Western Blotting.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Quality
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected MUTYH in 10 µg of Raji cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected MUTYH in 10 µg of Raji cell lysate.
Target description
~59 kDa observed. 60.07 kDa (isoform Alpha-1), 59.14 kDa (isoform Alpha-2), 59.07 kDa (isoform Alpha-3), 58.44 kDa (isoform Beta-1), 57.52 kDa (isoform Gamma-2), and 50.52 kDa (isoform Gamma-3) calculated.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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