antibody form
purified antibody
Quality Level
clone
dG9
monoclonal
purified by
using protein G
species reactivity
mouse, human
concentration
(Please refer to lot specific datasheet.)
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
isotype
IgG2bκ
UniProt accession no.
General description
Purified granules from the human cytotoxic large granular lymphocytes lymphoma line YT corresponding to human Perforin-1.
Specificity
Reacts with non-denatured human perforin precursor and mature perforin, but not denatured perforin.
Application
Immunoprecipitation Analysis: A representative lot immunoprecipitated Perforin-1 in YT cell lysate with or without Endo H treatment (Uellner, R., et al. (1997). EMBO. 16(24):7287-7296).
Immunocytochemistry Analysis: A representative lot detected Perforin-1 in human lymphokine-activated killer (LAK) cells (Hameed, A., et al. (1992). American J. of Pathology. 140(5):1025-1030).
Immunohistochemistry Analysis: A representative lot detected Perforin-1 in human cardiac biopsy samples during allograft rejection (Hameed, A., et al. (1992). American J. of Pathology. 140(5):1025-1030).
Immunocytochemistry Analysis: A representative lot detected Perforin-1 in human lymphokine-activated killer (LAK) cells (Hameed, A., et al. (1992). American J. of Pathology. 140(5):1025-1030).
Immunohistochemistry Analysis: A representative lot detected Perforin-1 in human cardiac biopsy samples during allograft rejection (Hameed, A., et al. (1992). American J. of Pathology. 140(5):1025-1030).
Quality
Evaluated by Flow Cytometry in human peripheral blood mononuclear cells.
Flow Cytometry Analysis: 3.0 µg of this antibody detected Perforin-1 in human peripheral blood mononuclear cells.
Flow Cytometry Analysis: 3.0 µg of this antibody detected Perforin-1 in human peripheral blood mononuclear cells.
Physical properties
61 kDa calculated
Physical form
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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