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MABE286

Anti-Replication Protein A Antibody, clone RPA34-19

Name: Anti-Replication Protein A Antibody, clone RPA34-19
Brand: MM

clone RPA34-19, from mouse

Synonym(s):

Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, RF-A protein 2, Replication protein A 34 kDa subunit, RP-A p34

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

Pricing and availability is not currently available.

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

RPA34-19, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_002937

UniProt accession no.

P15927

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1 of 4

This Item	IDF00047	IDF00101	IDF00103
IDF00045 2,2′-Dihydroxychalcone	IDF00047 3,2′-Dihydroxychalcone	IDF00101 2,6′-Dimethoxy-2′-hydroxychalcone	IDF00103 3,4′-Dimethoxy-2′-hydroxychalcone
form solid	form solid	form solid	form solid

General description

RPA (RP-A p32) is a heterotrimeric protein complex that binds specifically to single-stranded DNA (ssDNA). It is composed of three subunits: RPA1 (70 kDa), RPA2 (32 kDa), and RPA3 (14 kDa). RPA plays multiple roles in DNA replication. At the onset of DNA replication, RPA is loaded onto chromatin, and is needed for subsequent loading of DNA polymerase and other replication proteins to initiate DNA replication. After replication begins, RPA moves with replication forks, stabilizing ssDNA and assisting in DNA synthesis. In addition to its replication function, RPA is also known to play essential roles in damage repair and recombination. The 32 kDa subunit is phosphorylated by the cdc2 family of kinases when cells enter S-phase; and by ATM, ATR, and DNA-PK proteins in response to DNA damage.

Immunogen

Replication Protein A purified from U293 cells.

Application

Anti-Replication Protein A Antibody, clone RPA34-19 is a Mouse Monoclonal Antibody for detection of Replication Protein A also known as Replication protein A 32 kDa subunit, RP-A p32 & has been validated in WB, ICC & IHC.

Immunocytochemistry Analysis: A 1:250 dilution from a reprsentative lot detected Replication Protein A in A431 cells.

Immunohistochemistry Analysis: A 1:5 dilution from a representative lot detected Replication Protein A in human placental chorionic villi and in human colorectal adenocarcinoma tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:2,000 dilution of this antibody detected Replication Protein A in 10 µg of HeLa cell lysate.

Target description

~34 kDa observed. This protein has 3 isoforms: Isoform 1 (~29 kDa), Isoform 2 (~30 kDa), and Isoform 3 (~39 kDa).

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Radiotherapy in poor risk patients with stage I cancer of the endometrium: results of not giving external beam radiotherapy.

B DeCruze et al.

Clinical oncology (Royal College of Radiologists (Great Britain)), 11(4), 252-254 (1999-09-04)

Poor prognosis (poorly differentiated and/or deep myometrial invasion) Stage I endometrial cancer can have a relapse rate as high as 50%. Traditionally, most clinical oncologists treat these patients with external beam radiotherapy after surgery but there is no evidence to

An imaging dataset of cervical cells using scanning near-field optical microscopy coupled to an infrared free electron laser.

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Scientific data, 4, 170084-170084 (2017-07-12)

Using a scanning near-field optical microscope coupled to an infrared free electron laser (SNOM-IR-FEL) in low-resolution transmission mode, we collected chemical data from whole cervical cells obtained from 5 pre-menopausal, non-pregnant women of reproductive age, and cytologically classified as normal

Theoretical study of the gas-phase reactions of iodine atoms ((2)P(3/2)) with H(2), H(2)O, HI, and OH.

Sébastien Canneaux et al.

The journal of physical chemistry. A, 114(34), 9270-9288 (2010-08-03)

The rate constants of the reactions of iodine atoms with H(2), H(2)O, HI, and OH have been estimated using 39, 21, 13, and 39 different levels of theory, respectively, and have been compared to the available literature values over the

Study on the determination and chiral inversion of R-salbutamol in human plasma and urine by liquid chromatography-tandem mass spectrometry.

Ting Zhou et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1002, 218-227 (2015-09-06)

The chiral inversion has been a concerned issue during the research and development of a chiral drug. In this study, a sensitive chiral liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for determination of salbutamol enantiomers in human plasma and

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