MABE659

Anti-Myb Antibody, clone 8F12.1

clone 8F12.1, from mouse

• Synonym(s): Transcriptional activator Myb, Proto-oncogene c-Myb
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 8F12.1, monoclonal
• species reactivity: human
• technique(s): western blot: suitable
• NCBI accession no.: NP_005366
• UniProt accession no.: P10242
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... MYB(4602)

Description

General description

Transcriptional activator Myb (UniProt: P10242; also known as Myb, Proto-oncogene c-Myb) is encoded by the MYB gene (Gene ID: 4602) in human. Myb is a transcriptional activator; DNA-binding protein that specifically recognize the sequence 5′-YAAC[GT]G-3′ and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. It is negatively regulated by microRNA-155. It consists of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain, and a C-terminal domain involved in transcriptional repression. MYB gene is aberrantly expressed or rearranged or undergo translocation in leukemias and lymphomas, and is considered to be an oncogene. Myb protein is also reported to bind to HTLV-I long terminal repeat (LTR) sequences at four distinct regions in a sequence specific manner and activates LTR-mediated transcription. (Ref.: Dasgupta, P., et al. (1992). J. Virol. 66(1): 270-276).

Specificity

Clone 8F12.1 specifically detects human Myb protein. It targets an epitope within 154 amino acids in the C-terminal region.

Immunogen

GST/His-taged recombinant protein fragment corresponding to 154 amino acids from the C-terminal region of human Transcriptional activator Myb.

Application

Anti-Myb, clone 8F12.1 Antibody, Cat. No. MABE659, is a highly specific mouse monoclonal antibody that targets Myb and has been tested in Western Blotting.

Research Category
Epigenetics & Nuclear Function

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Myb in 10 µg of Jurkat cell lysate.

Target description

~80 kDa observed; 72.34 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1