MABE940
Anti-phospho Histone H3 (Thr11) Antibody, clone 6G12C5
clone 6G12C5, 1 mg/mL, from rat
Synonym(s):
Histone H3.3
Sign Into View Organizational & Contract Pricing
All Photos(5)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rat
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6G12C5, monoclonal
species reactivity
human
concentration
1 mg/mL
technique(s)
ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pThr11)
Gene Information
human ... H3F3B(3021)
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Immunogen
KLH-conjugated linear peptide corresponding to human Histone H3 phosphorylated at Thr11.
Application
This Anti-phospho Histone H3 (Thr11) Antibody, clone 6G12C5 is validated for use in western blotting, dot blot, ELISA & ICC for the detection of phospho Histone H3 (Thr11).
Western Blotting Analysis: 1 µg/mL from a representative lot detected phospho Histone H3 (Thr11) in 10 µg of nocodazole treated HeLa cell lysate.
Dot Blot (Specificity) Analysis: 0.2 µg/mL from a representative lot detected phospho Histone H3 (Thr11) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected phospho Histone H3 (Thr11) in HeLa cells undergoing interphase and metaphase (Prof. Taro Tachibana, Cell Engineering Corporation.).
ELISA Analysis: A representative lot detected phospho Histone H3 (Thr11) in a panel of Histone H3 peptides (Prof. Taro Tachibana, Cell Engineering Corporation.).
Western Blotting Analysis: A representative lot detected phospho Histone H3 (Thr11) in HCT116 cell lysates, which were incubated for a period of time with either nocodazole or UV. The same lot detected phospho Histone H3 (Thr11) in HeLa cell lysates, which were incubated for a period of time with nocodazole. Additionally, the same lot detected phospho Histone H3 (Thr11) in tsFT210 whole cell extracts after released G2/M syncronization (Prof. Taro Tachibana, Cell Engineering Corporation.).
Western Blotting Analysis: A representative lot from an independent laboratory detected phospho Histone H3 (Thr11) in nocodazole treated HeLa extract (Yoshimi, T., et al. (2013). Monoclon Antib Immunodiagn Immunother. 32(2):119-124.)
ELISA Analysis: A representative lot from an independent laboratory detected phospho Histone H3 (Thr11) in a panel of various phosphorylated H3 peptides (Yoshimi, T., et al. (2013). Monoclon Antib Immunodiagn Immunother. 32(2):119-124.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected phospho Histone H3 (Thr11) in mitotic DM4 cells (Yoshimi, T., et al. (2013). Monoclon Antib Immunodiagn Immunother. 32(2):119-124.).
Dot Blot (Specificity) Analysis: 0.2 µg/mL from a representative lot detected phospho Histone H3 (Thr11) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected phospho Histone H3 (Thr11) in HeLa cells undergoing interphase and metaphase (Prof. Taro Tachibana, Cell Engineering Corporation.).
ELISA Analysis: A representative lot detected phospho Histone H3 (Thr11) in a panel of Histone H3 peptides (Prof. Taro Tachibana, Cell Engineering Corporation.).
Western Blotting Analysis: A representative lot detected phospho Histone H3 (Thr11) in HCT116 cell lysates, which were incubated for a period of time with either nocodazole or UV. The same lot detected phospho Histone H3 (Thr11) in HeLa cell lysates, which were incubated for a period of time with nocodazole. Additionally, the same lot detected phospho Histone H3 (Thr11) in tsFT210 whole cell extracts after released G2/M syncronization (Prof. Taro Tachibana, Cell Engineering Corporation.).
Western Blotting Analysis: A representative lot from an independent laboratory detected phospho Histone H3 (Thr11) in nocodazole treated HeLa extract (Yoshimi, T., et al. (2013). Monoclon Antib Immunodiagn Immunother. 32(2):119-124.)
ELISA Analysis: A representative lot from an independent laboratory detected phospho Histone H3 (Thr11) in a panel of various phosphorylated H3 peptides (Yoshimi, T., et al. (2013). Monoclon Antib Immunodiagn Immunother. 32(2):119-124.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected phospho Histone H3 (Thr11) in mitotic DM4 cells (Yoshimi, T., et al. (2013). Monoclon Antib Immunodiagn Immunother. 32(2):119-124.).
Quality
Evaluated by Western Blotting in nocodazole treated and untreated HeLa acid extract.
Western Blotting Analysis: 1 µg/mL of this antibody detected phospho Histone H3 (Thr11) in 10 µg of nocodazole treated HeLa acid extract.
Western Blotting Analysis: 1 µg/mL of this antibody detected phospho Histone H3 (Thr11) in 10 µg of nocodazole treated HeLa acid extract.
Target description
~17 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Linkage
Replaces: MABE468
Physical form
Format: Purified
Not finding the right product?
Try our Product Selector Tool.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
A panel of specific monoclonal antibodies directed against various phosphorylated histones H3.
Yoshimi, T; Ohkawa, Y; Azuma, M; Tachibana, T
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy null
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service