MABF2193
Anti-pan Dengue Virus (1-4) Antibody, Clone 323
clone 323
Synonym(s):
Dengue virus 1-4 NS1 protein, pan-DENV NS1 protein
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
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biological source
mouse
antibody form
purified antibody
clone
323, monoclonal
mol wt
~48 kDa (calculated for NS1 protein; 378.71 kDa for the entire genome polyprotein.)
purified by
using protein G
species reactivity
human
isotype
IgG1κ
UniProt accession no.
General description
Dengue virus (DENV) is a mosquito-borne flavivirus and is one of the most common mosquito-transmitted viral infections. The DENV particle is about 500 nm in diameter and includes a positive-sense RNA genome with ~10,700 nucleotides and 3 structural proteins known as capsid (C), precursor membrane (prM), and envelope (E) proteins. In addition, the RNA genome of dengue virus also encodes seven nonstructural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5) that are shown to be essential for viral replication. DENV NS1, a 48-kDa glycoprotein (aa 776-1127 of genome polyprotein), is highly conserved among all flaviviruses and is essential for viral replication. It is expressed as a monomer in infected cells and after post-translational modification in the lumen of endoplasmic reticulum, it forms homodimers associated with organelle membranes and the cell membrane. NS1 is reported to be involved in immune evasion, pathogenesis, and viral replication. Once cleaved off the polyprotein, is targeted to three destinations: the viral replication cycle, the plasma membrane and the extracellular compartment. NS1 is secreted in a hexamer form, which is composed of three dimers with a detergent-sensitive hydrophobic central cavity and it plays a role against host immune response. NS1 is shown to induce expression of sialidases, heparanase, and activates cathepsin L, which activates heparinase via enzymatic cleavage. These effects are may contribute to the endothelial hyperpermeability commonly observed in severe dengue disease. During the recovery phase, NS1 is cleared from the circulation by antibody-mediated effects. Clone 323 displays reactivity to NS1 protein in all Dengue viruses (1-4).
Specificity
Clone 323 is a mouse monoclonal antibody that detects all four serotypes of Dengue virus NS1 protein.
Immunogen
Purified recombinant Dengue virus NS1 protein expressed in mammalian cells.
Application
Flow Cytometry Analysis: A representative lot detected pan Dengue Virus (1-4) in Flow Cytometry applications
ELISA Analysis: A representative lot detected pan Dengue Virus (1-4) in ELISA applications
ELISA Analysis: A representative lot detected pan Dengue Virus (1-4) in ELISA applications
Quality
Evaluated by ELISA in DENV1, DENV2, DENV3, and DENV4 NS1 recombinant protein.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
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