MABF3205

Anti-CD205/Ly-75 Antibody, clone HD30

• Synonym(s): C-type lectin domain family 13 member B, DEC-205, Lymphocyte antigen 75, gp200-MR6
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: HD30, monoclonal
• mol wt: calculated mol wt 198.31 kDa; observed mol wt ~N/A kDa
• purified by: using protein G
• species reactivity: human, monkey
• packaging: antibody small pack of 100
• technique(s): flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: Extracellular domain
• Protein ID accession no.: NP_001185688
• UniProt accession no.: O60449
• storage temp.: 2-8°C
• Gene Information: human ... LY75(4065)

Description

Specificity

Clone HD30 is a mouse recombinant monoclonal antibody that detects CD205/Ly-75. It targets an epitope within the extracellular CRD1-2 subdomain.

Immunogen

Full-length, Fc-tagged, recombinant human CD205/Ly-75

Application

Quality Control Testing

Evaluated by Flow Cytometry in one million human peripheral blood mononuclear cells (PMBCs).

Flow Cytometry Analysis: 1.0 µg from a representative lot detected CD205/Ly-75 in one million human peripheral blood mononuclear cells (PMBCs).

Tested Applications

Flow Cytometry Analysis: A representative lot detected CD205/Ly-75 in Flow Cytometry application (Park, C.G., et al. (2012). J Immunol Methods. 377(1-2):15-22).

Immunohistochemistry Applications: A representative lot detected CD205/Ly-75 in Immunohistochemistry application (Park, C.G., et al. (2012). J Immunol Methods. 377(1-2):15-22).

Western Blotting Analysis: A representative lot detected CD205/Ly-75 in Western Blotting application (Park, C.G., et al. (2012). J Immunol Methods. 377(1-2):15-22).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Lymphocyte antigen 75 (UniProt: O60449; also known as CD205, Ly-75, C-type lectin domain family 13 member B, DEC-205, gp200-MR6) is encoded by the LY75 (also known as CD205, CLEC13B) gene (Gene ID: 4065) in human. It belongs to the to the C-type multilectin family. Its a putative endocytic receptor specific to the uptake and processing of captured antigens for dendritic cells (DC), and is especially known for its expression and presence on the cell surface of CD8+ DCs. CD8+CD205+ DCs are unique in that they seem to be specialized to the uptake of apoptotic cells, in their ability to resist certain viral infections, and their apparent specialization for cross-presentation. CD205/DEC205 is a single-pass type I membrane protein, which is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 28-1666), a transmembrane domain (aa 1667-1691), and a cytoplasmic domain (aa 1692-1722). The large extracellular domain consists of a number of subdomains, including a cysteine rich (CR) domain, a fibronectin type II (FN) domain, and 10 contiguous carbohydrate recognition domains (CRDs). CD205 is predominantly expressed on dendritic cells, colon, spleen, thymus and peripheral blood lymphocytes, and also found in both myeloid and B lymphoid cell lines. Splice variants are known to produce 5 isoforms. Isoforms 2 and 3 are of interest in the study of malignant Hodgkin lymphoma cells or HRS cells. CD205 has been implicated in various diseases, particularly in the context of cancer and autoimmune disorders where aberrant expression or function of CD205 may contribute to dysregulated immune responses. It is also a target of interest in therapeutic drug research for Epstein-Barr virus. (Ref.: Park, C.G., et al. (2012). J Immunol Methods. 377(1-2):15-22).

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.