MABF3373

Anti-APOBEC3A/B/G Antibody, clone 04A04

• Synonym(s): A3A, APOBEC-3B, APOBEC-3G, DNA dC->dU-editing enzyme APOBEC-3A, EC:3.5.4.38, Phorbolin-1, Phorbolin-1-related protein, Phorbolin-2/3
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 04A04, monoclonal
• mol wt: calculated mol wt 23 kDa; observed mol wt ~18 kDa
• purified by: using protein G
• species reactivity: human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 100
• technique(s): western blot: suitable
• isotype: IgG1κ
• epitope sequence: C-terminal
• Protein ID accession no.: NP_001257340 / NP_001180218 / NP_068594
• UniProt accession no.: Q9UH17 / P31941 / Q9HC16
• storage temp.: 2-8°C

Description

Specificity

Clone 04A04 is a mouse recombinant monoclonal antibody that detects APOBEC3A/B/G. It targets an epitope within 29 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 29 amino acids from the C-terminal of human APOBEC3B.

Application

Quality Control Testing

Evaluated by Western Blotting in BT-474 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected APOBEC3A/B in BT-474 cell lysate.

Tested Applications

Western Blotting Analysis: A representative lot detected APOBEC3A/B/G in Western Blotting application (Petljak, M., et al. (2022). Nature. 607(7920):799-807; Dananberg, A., et al. (2024). Methods Cell Biol. 182:313-327).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

DNA dC->dU-editing enzyme APOBEC-3A/-3B/-3G (UniProt: P31941/Q9UH17/Q9HC16) is encoded by the APOBEC-3A/-3B/-3G gene (Gene ID: 200315/9582/) in human. The APOBEC3 family of cytosine deaminases catalyze the deamination of cytidine to uridine in single-stranded DNA and RNA. There are seven APOBEC3 enzymes: A3A, A3B, A3C, A3D, A3F, A3G, and A3H. They play essential roles in innate immunity by restricting by restricting the replication of various viruses. On the other hand, their aberrant activities can drive mutagenesis of virus and cancer genomes. A3 proteins have been implicated in the evolution of viruses, causing severe manifestations such as drug resistance and/or immune evasion. (Ref: Jonathan, M., and Ikeda, T., (2023), Front. Virol., 3:1332010)

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.