MABF737
Anti-Ly-6G Antibody (mouse), FITC, clone 1A8
clone 1A8, 0.5 mg/mL, from rat
Synonym(s):
Lymphocyte antigen 6G, Ly-6G, Ly-6G.1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rat
Quality Level
conjugate
FITC conjugate
antibody form
purified antibody
antibody product type
primary antibodies
clone
1A8, monoclonal
species reactivity
mouse
packaging
antibody small pack of 25 μg
concentration
0.5 mg/mL
technique(s)
flow cytometry: suitable
General description
The 1A8 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the 1A8 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes. Note: for identification of Ly-C, an alternative antibody, clone RB6-8C5, has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C.
This rat monoclonal antibody Anti-Ly-6G (mouse), FITC & clone 1A8 validated for use in Flow Cytometry for the detection of Ly-6G.
Quality
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.25 µg of this antibody detected Ly-6G in one million C57Bl/6 bone marrow cells.
Flow Cytometry Analysis: 0.25 µg of this antibody detected Ly-6G in one million C57Bl/6 bone marrow cells.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
Certificates of Analysis (COA)
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