MABF950

Anti-Integrin alpha-4 (CD49d) Antibody, clone TA-2

clone TA-2, from mouse

• Synonym(s): Protein Itga4, CD49d, IA4, VLA-4 alpha
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: TA-2, monoclonal
• species reactivity: rat
• technique(s): flow cytometry: suitable; immunoprecipitation (IP): suitable
• isotype: IgG1κ
• UniProt accession no.: D3ZMQ3
• shipped in: ambient

Description

General description

Integrin alpha-4 (UniProt: D3ZMQ3; also known as CD49d, Protein Itga4) is encoded by the Itga4 gene in rat. Integrin-Alpha 4 contains 7 GF-GAP repeats. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. It associates with either beta-1 or beta-7 to generate a heterodimer. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Integrin alpha-4/beta-1 (alpha 4/CD29; VLA-4) may regulate leukocyte to endothelial cells adhesion by controlling Junctional adhesion molecules-L homodimerization. Integrins alpha-4/beta-1 and alpha-4/beta-7 are receptors for fibronectin and they recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-4/beta-1 plays a major role in the regulation of immune cell recruitment to inflamed endothelia and sites of inflammation. On activated endothelial cells integrin alpha-4/beta-1 triggers homotypic aggregation for most alpha-4/beta-1-positive leukocyte cell lines. This monoclonal antibody (clone TA-2) is shown to inhibit rat lymphocyte adhesion to stimulated HUVEC. It also blocks adhesion small peritoneal exudate lymphocytes (sPEL) adhesion to TNF-alpha and IL-1 stimulated human umbilical vein endothelial cells. (Ref.: Issekutz, TB., and Wykretowicz, A (1991). J. Immunol. 147:109-116).

Specificity

Clone TA-2 detects CD49d/Integrin alpha-4 in Rat splenocytes and lymphocytes.

Immunogen

Small peritoneal exudate lymphocytes (sPELs).

Application

Flow Cytometry Analysis: A representative lot detected Integrin alpha-4 (CD49d) in Flow Cytometry appications (Latta, M., et. al. (2007). Immunology. 121(4):555-64).

Flow Cytometry Analysis: A representative lot detected Integrin alpha-4 (CD49d) in Flow Cytometry appications (Schlager, C., et. al. (2016). Nature. 530(7590):349-53).

Agonist or Inhibitor Analysis: A representative lot detected Integrin alpha-4 (CD49d) in Agonist or Inhibitor appications (Issekutz, T.B., et. al. (1991). J Immunol. 147(12):4178-84).

Agonist or Inhibitor Analysis: A representative lot detected Integrin alpha-4 (CD49d) in Agonist or Inhibitor appications (Odoardi, F., et. al. (2012). Nature. 488(7413):675-9).

Agonist or Inhibitor Analysis: A representative lot detected Integrin alpha-4 (CD49d) in Agonist or Inhibitor appications (Schlager, C., et. al. (2016). Nature. 530(7590):349-53).

Immunoprecipitation Analysis: A representative lot detected Integrin alpha-4 (CD49d) in Immunoprecipitation appications (Schlager, C., et. al. (2016). Nature. 530(7590):349-53).

This mouse monoclonal Anti-Integrin alpha-4 (CD49d), clone TA-2, Cat. No. MABF950 is tested for use in Flow Cytometry, Immunoprecipitation, and in functional studies as Agonist or Inhibitor for the detection of CD49d/Integrin alpha-4.

Quality

Evaluated by Flow Cytometry in rat splenocytes and lymphocytes.

Flow Cytometry Analysis: 1 µg of this antibody detected Integrin alpha-4 (CD49d) in one million rat splenocytes and lymphocytes.

Target description

115.59 kDa calculated.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable