MABN221

Anti-NCAM1 Antibody, clone 11G7.1

clone 11G7.1, from mouse

• Synonym(s): Neural cell adhesion molecule 1, N-CAM-1, NCAM-1, CD56
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 11G7.1, monoclonal
• species reactivity: rat, mouse
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001074914
• UniProt accession no.: P13595

Description

General description

The Immunoglobulin superfamily of cell adhesion molecules is related to the immunoglobulin proteins by structure. The family contains over a 100 members, which include ICAM (intercellular adhesion molecule), NCAM (neural cell adhesion molecule) and NgCAM (neural-glial cell adhesion molecule). Both NCAM and NgCAM are calcium independent adhesion molecules. NCAM, the first CAM to be characterized, is expressed on all neurons from very early in development. It binds homophilically to NCAM on adjacent cells and has a variety of functions during neural evelopment. One of these important functions is to hold developing neurites together during neuron outgrowth and formation of neural connections. The adhesive properties of NCAM are modulated during development and plasticity by the presence of the regulated carbohydrate, polysialic acid (PSA). The post-translational addition of alpha-2,8-linked PSA (NCAM-PSA) appears to contribute to synaptic plasticity during learning and memory formation and to the mechanisms of nerve regeneration.

Specificity

This antibody recognizes the cytoplasmic domain of NCAM1.

Immunogen

Epitope: cytoplasmic domain

GST-tagged recombinant protein corresponding to the cytoplasmic domain of mouse NCAM1.

Application

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

This NCAM1 antibody is validated for use in WB & IHC for the detection of the NCAM1 protein.

Western Blot Analysis: 0.5 µg/mL from a representative lot detected NCAM1 in 10 µg of mouse brain tissue lysate.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected NCAM1 in rat hippocampus and rat cerebral cortex tissues.

Quality

Evaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected NCAM1 in 10 µg of rat brain tissue lysate.

Target description

~180 kDa observed. Uniprot describes 3 isoforms produced by alternative splicing at ~94 kDa, ~80 kDa, and ~67 kDa The calculated molecular weight of this protein is 120 kDa, but can be observed between ~105-200 kDa due to high glycosylation (Patel, K., et al. (1991). Br J Cancer Suppl. 14:20-13).

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable