MABS1135

Anti-GNAQ Antibody, clone 8A11.1

clone 8A11.1, from mouse

• Synonym(s): Guanine nucleotide-binding protein G(q) subunit alpha, Guanine nucleotide-binding protein alpha-q
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 8A11.1, monoclonal
• species reactivity: human, rat
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_002063
• UniProt accession no.: P50148

Description

General description

Guanine nucleotide-binding protein G(q) subunit alpha (UniProt P50148; also known as Guanine nucleotide-binding protein alpha-q) is encoded by the GNAQ (also known as GAQ, SWS) gene (Gene ID 2776) in human. Heterotrimeric G proteins are activated by G protein-coupled receptors (GPCRs) and are composed of alpha (α), beta (β), and gamma (γ) subunits. There exists also monomeric Ras superfamily of small GTPases that are commonly referred to as small G-proteins. G-proteins mediate signal transduction initiated by GPCRs. Ligand binding activates GPCRs by inducing a conformational change. The activated GPCRs catalyzes the GDP-to-GTP exchange of the Gα subunit, causing a dissociation of the Gβγ dimer from the now GTP-bound Gα subunit. The Gα subunit gradually hydrolyzes the bound GTP to GDP and eventually re-associates with Gβγ and re-starts the cycle. There exist at least 20 different Gα subunits, which are separated into four main groups, G1, Gs, Gq, and G12/13. Four different genes code for Gq alpha subunits, GNAQ, GNA11, GNA14, and GNA15. Gq proteins mediate a variety of cellular signaling events by activating phospholipase C (PLC). Gq-coupled GPCRs include α1-adrenoreceptor, angiotensin II receptor type 1, calcitonin receptor, muscarinic receptors (M1, M3, and M5), histamine H1 receptor, Serotonin 5-HT2 receptors, vasopressin type 1 receptors 1A and 1B.

Specificity

Clone 8A11.1 was raised against a human Gqα (GNAQ) recombinant fragment. Clone 8A11.1 reactivity toward Gq/11α (GNA11), Gq/14α (GNA14), and Gq/15α (GNA15) has not been tested.

Immunogen

Epitope: Internal.

GST-tagged recombinant internal fragment of human GNAQ.

Application

Anti-GNAQ Antibody, clone 8A11.1 is an antibody against GNAQ for use in Western Blotting, Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected GNAQ in rat testis, human prostate, human small intestine, and human prostate cancer tissue sections.

Research Category
Signaling

Research Sub Category
G-proteins

Quality

Evaluated by Western Blotting in human testis tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected GNAQ in 10 µg of human testis tissue lysate.

Target description

~40 kDa observed. 42.14 kDa calculated. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable