MABT1571

Anti-KIAA0753 Antibody, clone 5H3

• Synonym(s): OFD1- and FOPNL-interacting protein, Protein moonraker
• UNSPSC Code: 12352203

Properties

• biological source: rat
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 5H3, monoclonal
• mol wt: calculated mol wt 109.41 kDa; observed mol wt ~120 kDa
• purified by: using protein G
• species reactivity: human
• packaging: antibody small pack of 100
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG2a
• epitope sequence: C-terminal
• Protein ID accession no.: NP_055619
• UniProt accession no.: Q2KHM9
• storage temp.: 2-8°C
• Gene Information: human ... KIAA0753(9851)

Description

Specificity

Clone 5H3 is a rat monoclonal antibody that detects KIAA0753. It targets an epitope within 301 amino acids from the C-terminal region.

Immunogen

GST-tagged recombinant protein corresponding to 301 amino acids from the C-terminal region of human KIAA0753.

Application

Quality Control Testing

Evaluated by Western Blotting in K-562 cell lysate.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected KIAA0753 in K-562 cell lysate.

Tested Applications

Immunofluorescence Analysis: A representative lot detected KIAA0753 in immunofluorescence application (Chevrier, V., et al. (2016). Hum Mol Genet. 25(3):497-513; Kumar, D., et al. (2021). J Cell Biol. 220(9):e202011133).

Immunohistochemistry Analysis: A representative lot detected KIAA0753 in Immunohistochemistry application (Chevrier, V., et al. (2016). Hum Mol Genet. 25(3):497-513).

Western Blotting Analysis: A representative lot detected KIAA0753 in Western Blotting application (Chevrier, V., et al. (2016). Hum Mol Genet. 25(3):497-513).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Protein moonraker (UniProt: Q2KHM9; also known as MNR, OFD1- and FOPNL-interacting protein ) is encoded by the KIAA0753 (also known as MNR, OFIP) gene (Gene ID: 9851) in human. KIAA0753 is one of the key regulators of mother centriole function, essential for normal vertebrate development, Hedgehog signaling, and ciliogenesis. I also plays a role in microtubule stabilization. KIAA0753 with OFD1 and CEP90 forms an evolutionarily conserved complex, named DISCO (distal centriole complex). DISCO acts at the distal centriole and by restraining centriole length it supports ciliogenesis and assembling distal appendages. Mutations in KIAA0753 have been implicated in ciliopathies. These include conditions such as skeletal dysplasia, Joubert and Orofaciodigital (OFD) syndrome. (Ref.: Kumar, D., et al. (2021). J Cell Biol. 220(9):e202011133)

Physical form

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.