MABT1586
Anti-Profilin 1 Antibody, clone 2C5
Synonym(s):
Profilin-1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2C5, monoclonal
mol wt
calculated mol wt 14.96 kDa
observed mol wt ~15 kDa
purified by
using protein G
species reactivity
mouse
packaging
antibody small pack of 100 μg
General description
Profilin-1 (UniProt: P62962; also known as Profilin I) is encoded by the Pfn1 gene (Gene ID: 18643) in murine species. Five profilin isoforms have been identified in mammals: Profilins I, IIa, IIb, III and IV. They regulate actin dynamics at plasma membranes during the assembly, maintain and disassemble actin networks at the leading edge of locomoting animal cells during cytokinesis, and are involved in embryonic development and morphogenesis. Profilins bind to G-actin in a 1:1 complex and thereby sequesters monomeric actin. Profilin I is highly expressed in all tissues. It binds to actin and affects the structure of the cytoskeleton. In neurons, proper profilin 1 function is essential during development and is implicated in the formation and maintenance of the neuronal cytoskeleton, growth-cone formation, synaptogenesis, synaptic activities, shape and morphological dynamics, and the growth of dendrites and axons. Knockdown of both profilins I and II is reported to inhibit neurite outgrowth, possibly impairing actin dynamics by reducing the availability of profilin-G-actin complexes for microfilament growth. Higher levels of profilin 1 have been reported in subjects with severe aplastic anemia. G118V and C71G mutations in profilin 1 have been linked to Amyotrophic lateral sclerosis (ALS). (Ref.: Yu, H., et al. (2021). Front. Immunol. 12; 631954; Kaiei, M., et al. (2018). Sci. Rep. 8; Article 13102; Murk, K., et al. (2012). PLoS One. 7(3); e34167; Jockusch, BM., et al. (2007). Rev. Physiol. Biochem. Pharmacol. 159; 131-149).
Specificity
Clone 2C5 is a mouse monoclonal antibody that detects Profilin 1. It targets an epitope within 14 amino acids from the N-terminal region.
Immunogen
A linear peptide corresponding to 14 amino acids from the N-terminal region of mouse Profilin-1.
Application
Quality Control Testing
Evaluated by Western Blotting in Mouse spleen tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Profilin 1 in Mouse spleen tissue lysate.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected Profilin-1 in Immunocytochemistry applications (Murk, K., et al. (2012). PLoS One. 7(3); e34167).
Western Blotting Analysis: A representative lot detected Profilin-1 in Western Blotting applications (Murk, K., et al. (2012). PLoS One. 7(3); e34167).
Immunofluorescence Analysis: A representative lot detected Profilin-1 in Immunofluorescence applications (Murk, K., et al. (2012). PLoS One. 7(3); e34167).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Mouse spleen tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Profilin 1 in Mouse spleen tissue lysate.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected Profilin-1 in Immunocytochemistry applications (Murk, K., et al. (2012). PLoS One. 7(3); e34167).
Western Blotting Analysis: A representative lot detected Profilin-1 in Western Blotting applications (Murk, K., et al. (2012). PLoS One. 7(3); e34167).
Immunofluorescence Analysis: A representative lot detected Profilin-1 in Immunofluorescence applications (Murk, K., et al. (2012). PLoS One. 7(3); e34167).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Profilin 1, clone 2C5, Cat. No. MABT1586, is a mouse monoclonal antibody that detects Profilin 1 and is tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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