MABT1586

Anti-Profilin 1 Antibody, clone 2C5

• Synonym(s): Profilin-1
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2C5, monoclonal
• mol wt: calculated mol wt 14.96 kDa; observed mol wt ~15 kDa
• purified by: using protein G
• species reactivity: mouse
• packaging: antibody small pack of 100 μg
• technique(s): immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: N-terminal
• Protein ID accession no.: NP_035202
• UniProt accession no.: P62962
• shipped in: 2-8°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Pfn1(18643)

Description

General description

Profilin-1 (UniProt: P62962; also known as Profilin I) is encoded by the Pfn1 gene (Gene ID: 18643) in murine species. Five profilin isoforms have been identified in mammals: Profilins I, IIa, IIb, III and IV. They regulate actin dynamics at plasma membranes during the assembly, maintain and disassemble actin networks at the leading edge of locomoting animal cells during cytokinesis, and are involved in embryonic development and morphogenesis. Profilins bind to G-actin in a 1:1 complex and thereby sequesters monomeric actin. Profilin I is highly expressed in all tissues. It binds to actin and affects the structure of the cytoskeleton. In neurons, proper profilin 1 function is essential during development and is implicated in the formation and maintenance of the neuronal cytoskeleton, growth-cone formation, synaptogenesis, synaptic activities, shape and morphological dynamics, and the growth of dendrites and axons. Knockdown of both profilins I and II is reported to inhibit neurite outgrowth, possibly impairing actin dynamics by reducing the availability of profilin-G-actin complexes for microfilament growth. Higher levels of profilin 1 have been reported in subjects with severe aplastic anemia. G118V and C71G mutations in profilin 1 have been linked to Amyotrophic lateral sclerosis (ALS). (Ref.: Yu, H., et al. (2021). Front. Immunol. 12; 631954; Kaiei, M., et al. (2018). Sci. Rep. 8; Article 13102; Murk, K., et al. (2012). PLoS One. 7(3); e34167; Jockusch, BM., et al. (2007). Rev. Physiol. Biochem. Pharmacol. 159; 131-149).

Specificity

Clone 2C5 is a mouse monoclonal antibody that detects Profilin 1. It targets an epitope within 14 amino acids from the N-terminal region.

Immunogen

A linear peptide corresponding to 14 amino acids from the N-terminal region of mouse Profilin-1.

Application

Quality Control Testing

Evaluated by Western Blotting in Mouse spleen tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Profilin 1 in Mouse spleen tissue lysate.

Tested Applications

Immunocytochemistry Analysis: A representative lot detected Profilin-1 in Immunocytochemistry applications (Murk, K., et al. (2012). PLoS One. 7(3); e34167).

Western Blotting Analysis: A representative lot detected Profilin-1 in Western Blotting applications (Murk, K., et al. (2012). PLoS One. 7(3); e34167).

Immunofluorescence Analysis: A representative lot detected Profilin-1 in Immunofluorescence applications (Murk, K., et al. (2012). PLoS One. 7(3); e34167).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Profilin 1, clone 2C5, Cat. No. MABT1586, is a mouse monoclonal antibody that detects Profilin 1 and is tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable