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MABT356

Sigma-Aldrich

Anti-Integrin alpha6 Antibody, clone 6H7.1

clone 6H7.1, from mouse

Synonym(s):

Integrin alpha-6, CD49 antigen-like family member F, VLA-6, CD49f

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Pricing and availability is not currently available.

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6H7.1, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

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This Item
922799250475267
technique(s)

ICP: suitable

technique(s)

ICP: suitable

technique(s)

ICP: suitable

technique(s)

ICP: suitable

grade

certified reference material, TraceCERT®

grade

certified reference material, TraceCERT®

grade

certified reference material, TraceCERT®

grade

certified reference material, TraceCERT®

format

single component solution

format

single component solution

format

single component solution

format

single component solution

concentration

1 g/L Gd in nitric acid (nominal concentration)

concentration

1 g/L Sc in nitric acid (nominal concentration)

concentration

10 g/L Sc in nitric acid (nominal concentration)

concentration

1 g/L Sr in nitric acid (nominal concentration)

application(s)

industrial qc
pharmaceutical

application(s)

industrial qc
pharmaceutical

application(s)

industrial qc
pharmaceutical

application(s)

industrial qc
pharmaceutical

product line

TraceCERT®

product line

TraceCERT®

product line

TraceCERT®

product line

TraceCERT®

General description

Integrin alpha-6 (UniProt P23229; also known as CD49 antigen-like family member F, CD49f, Integrin alpha6B, VLA-6) is encoded by the ITGA6 (also known as ITGA6B) gene (Gene ID 3655) in human. The biological functions attributed to cell-extracellular matrix (ECM) interactions are mediated primarily by integrin family transmembrane receptors. A total of 18 alpha and 8 beta integrins have been identified in human. Each alpha integrin pairs up with one beta integrin non-covalently to form a functional heterodimeric dimer. At least 24 distinct integrin heterodimers are known with varying ligand specificities. For example, the alpha6-beta1 integrin is reported to mediate the spreading and laminin-binding of Schwann cells, while the alpha6-beta4 integrin is shown to play a role in the suppression of anoikis in human intestinal epithelial cells (IECs).

Signal (1-23), Integrin alpha-6 (24-1130). Processed into Integrin alpha-6 heavy chain (24 – 938), Integrin alpha-6 light chain (942-1130), Processed integrin alpha-6 (Alpha6p; 636 – 1130)

Specificity

Expected to react with Integrin alpha-6 spliced isoforms 2 (Alpha-6X1A), 4 (Alpha-6X2A ), 6 (Alpha-6X1X2A), 7, and 9, as well as their respective proforms, cleaved light chains, and processed integrin alpha-6 forms (Alpha6p). Epitope is not present in isoform 1 (Alpha-6X1X2B), 3 (Alpha-6X1B), or 5 (Alpha-6X2B).

Immunogen

Epitope: Cytoplasmic region of isoforms 2/4/6/7/9
KLH-conjugated linear peptide corresponding to the cytoplasmic region of human Integrin alpha6 isoforms 2/4/6/7/9.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Integrin alpha6 in human skin tissue.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
This Anti-Integrin alpha6 Antibody, clone 6H7.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Integrin alpha6.

Quality

Evaluated by Western Blotting in SW480 human colon cancer cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Integrin alpha6 in 10 µg of SW480 human colon cancer cell lysate.

Target description

~140 kDa observed. Due to glycosylation, the target band(s) appears larger than the calculated 106.6 to 124.0 kDa based on the proform sequences of isoforms 2/4/6/7/9. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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    Violetta Sączyńska et al.
    Virology journal, 15(1), 13-13 (2018-01-18)
    The highly pathogenic avian influenza viruses of the H5 subtype, such as the H5N1 viral strains or the novel H5N8 and H5N2 reassortants, are of both veterinary and public health concern worldwide. To combat these viruses, monoclonal antibodies (mAbs) against
    Selvaraj Pavulraj et al.
    Veterinary microbiology, 210, 188-196 (2017-11-07)
    Equine influenza viruses (EIVs) are responsible for acute contagious respiratory infection in equines and the disease remains a major threat for equine population throughout the world despite vaccination strategies in place. The present study was aimed to assess the suitability
    Sapna Halder et al.
    Cell research, 15(9), 739-744 (2005-10-11)
    Rheumatoid synovial fibroblasts were used as an immunogen to produce monoclonal antibodies selected for their reactivity with stromal cell antigens. Mice were immunised with low passage whole cell preparations and the subsequent hybridomas were screened by immunohistochemistry on rheumatoid synovium
    Jorma Hinkula et al.
    Vaccines, 7(3) (2019-07-19)
    Background: Vaccination is commonly used to prevent and control influenza infection in humans. However, improvements in the ease of delivery and strength of immunogenicity could markedly improve herd immunity. The aim of this pre-clinical study is to test the potential
    Generation and characterization of novel stromal specific antibodies
    Halder S, et al.
    Cell Research, 15(9), 739-739 (2005)

    Protocols

    ELISA protocols providing detailed instructions on performing indirect ELISA and Capture ELISA . Learn more about recommended products and techniques for performing both sandwich ELISA and indirect ELISA experiments.

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