MABT883

Anti-CD315 Antibody, clone 17H1

clone 17H1, from rat

• Synonym(s): CD9 partner 1, CD9P1, Glu-Trp-Ile EWI motif-containing protein F, nEWI-F, Prostaglandin F2-alpha receptor regulatory protein, Prostaglandin F2-alpha receptor-associated protein
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 17H1, monoclonal
• species reactivity: human
• technique(s): flow cytometry: suitable; immunohistochemistry: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_065173.2
• UniProt accession no.: Q9P2B2
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... PTGFRN(5738)

Description

General description

Prostaglandin F2 receptor negative regulator (UniProt: Q9P2B2; also known as CD9 partner 1, CD9P1; Glu-Trp-Ile EWI motif-containing protein F; nEWI-F; Prostaglandin F2-alpha receptor regulatory protein; Prostaglandin F2-alpha receptor-associated protein; CD315) is encoded by the PTGFRN (also known as CD9P1, EWIF, FPRP, KIAA1436) gene (Gene ID 5738 ) in human. CD315 inhibits the binding of prostaglandin F2-alpha (PGF2-alpha) to its specific prostaglandin F2 alpha receptor, by decreasing the receptor number rather than the affinity constant. It is also reported to complex with 4-TM CD9 and CD81 and link them to the intracellular actin cytoskeleton. Its extracellular domain (ECD) contains six C2-type Ig-like domains (aa22-813) plus a Pro-Thr-Glu-Leu ER retention signal segment.

Specificity

Clone 17H1 detects CD315 in A549 cells by Flow cytometry.

Immunogen

Epitope: extracellular domain

Mesothelioma cell-derived exosomes.

Application

Flow Cytometry Analysis: 1:100 dilution of this antibody from a representative lot detected CD315 in one million U373 glioblastoma cells (Courtesy of Reinhard Zeidler, Helmholtz Zentrum Muenchen, Neuherberg, Germany in cooperation with Dr. A. Bankfalvi, Inst. für Pathologie Universitätsklinkum, Essen).

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected CD315 in Squamous cell carcinoma tissue (Courtesy of Reinhard Zeidler, Helmholtz Zentrum Muenchen, Neuherberg, Germany in cooperation with Dr. A. Bankfalvi, Inst. für Pathologie Universitätsklinkum, Essen).

Research Category
Cell Structure

This rat monoclonal Anti-CD315 antibody, clone 17H1, Cat. No. MABT883 is validated for use in Flow Cytometry and Immunohistochemistry for the detection of CD315.

Quality

Evaluated by Flow Cytometry in A549 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected CD315 in one million A549 cells.

Target description

98.55 kDa calculated

Physical form

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1