11-0398
Fluorescamine
≥98.0%
Synonym(s):
4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione
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About This Item
Empirical Formula (Hill Notation):
C17H10O4
CAS Number:
Molecular Weight:
278.26
Beilstein/REAXYS Number:
921143
EC Number:
MDL number:
UNSPSC Code:
12191502
PubChem Substance ID:
assay:
≥98.0%
Recommended Products
assay
≥98.0%
availability
available only in Japan
dilution
(for analytical testing)
mp
153-157 °C (lit.)
SMILES string
O=C1OC2(OC=C(C2=O)c3ccccc3)c4ccccc14
InChI
1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H
InChI key
ZFKJVJIDPQDDFY-UHFFFAOYSA-N
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Application
Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analyses.
Suitability
for HPLC
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Wen-Hsien Tsai et al.
Journal of chromatography. A, 1217(49), 7812-7815 (2010-11-04)
A simple sugaring-out assisted liquid-liquid extraction method combined with high-performance liquid-chromatography with fluorescence detection (HPLC-FL) was developed for the extraction and determination of sulfonamides in honey. Sample preparation consisted of acid hydrolysis to release sugar-bound sulfonamides. After derivatization with fluorescamine
Manjeet Deshmukh et al.
Biomaterials, 31(26), 6675-6684 (2010-06-22)
Two vinyl sulfone functionalized crosslinkers were developed for the purpose of preparing degradable poly(ethylene glycol) (PEG) hydrogels (EMXL and GABA-EMXL hydrogels). A self-elimination degradation mechanism in which an N-terminal residue of a glutamine is converted to pyroglutamic acid with subsequent
Laura Contreras-Ruiz et al.
Cornea, 29(5), 550-558 (2010-03-26)
Hyaluronic acid-chitosan nanoparticles (HA-CS NPs) have the potential to serve as a reliable drug delivery system to topically treat ocular surface disorders. We evaluated the in vivo uptake by ocular structures, the acute tolerance, and possible alterations of tear film
Grégoire Danger et al.
Electrophoresis, 29(19), 4036-4044 (2008-10-30)
The first results of chiral separations with the gradient elution isotachophoresis method are presented. As previously described, citrate is used in the run buffer as the leading ion and borate in the sample buffer as the terminating ion. Modulation of
José Bernal et al.
Journal of chromatography. A, 1216(43), 7275-7280 (2009-06-10)
A novel method for the simultaneous analysis at trace level of sulfonamides (sulfaguanidine, sulfanilamide, sulfacetamide, sulfathiazole, sulfapyridine, sulfachloropyridazine, sulfamerazine, sulfameter, sulfamethazine, sulfadoxine, sulfadiazine, sulfamonomethoxine, sulfadimethoxine) in honey is described. Methanol has been used in the sample treatment step to avoid
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