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A8097

Sigma-Aldrich

Acridine Orange hydrochloride solution

>95.0% purity (HPLC), solution

Synonym(s):

3,6-Bis(dimethylamino)acridine hydrochloride

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About This Item

Empirical Formula (Hill Notation):
C17H19N3 · HCl
CAS Number:
Molecular Weight:
301.81
Beilstein/REAXYS Number:
3575587
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

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Product Name

Acridine Orange hydrochloride solution, 10 mg/mL in H2O, ≥95.0% (HPLC)

Quality Level

assay

≥95.0% (HPLC)

form

solution

concentration

10 mg/mL in H2O

technique(s)

microbe id | staining: suitable

color

orange to red

εmax

47-54 at 486-492 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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1 of 4

This Item
14-602-M14-604-M14-790-M
biological source

human

biological source

human

biological source

human

biological source

-

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

mol wt

Mw 124 kDa (p110β), Mw 83.7 kDa (p85&alpha)

mol wt

Mw 125.3 kDa (p110α), Mw 83.7 kDa (p85α)

mol wt

Mw 121.6 kDa (P110δ), Mw 83.7 kDa (p85α)

mol wt

Mw 125.3 kDa (p110α), Mw 66.0 kDa (p65α)

UniProt accession no.

P42338

UniProt accession no.

P42336

UniProt accession no.

O00329

UniProt accession no.

P42336

NCBI accession no.

NM_006219.1

NCBI accession no.

NM_006218.2

NCBI accession no.

NM_005026.2

NCBI accession no.

NM_006218.2

Application

Acridine Orange hydrochloride solution has been used to study autophagic cell death. It has also been used for the staining of chromosomes.

Biochem/physiol Actions

Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentration it intercalates into DNA and precipitates RNA. However, at high concentration it denatures and precipitates both RNA and DNA. Acridine Orange is also used to analyze autophagy. It goes into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in the acidic organelle giving a bright red fluorescence.

pictograms

Health hazard

signalword

Warning

hcodes

Hazard Classifications

Muta. 2

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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