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C3023

Sigma-Aldrich

CHAPS hydrate

≥98% (HPLC)

Synonym(s):

3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS

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About This Item

Empirical Formula (Hill Notation):
C32H58N2O7S · xH2O
CAS Number:
331717-45-4
Molecular Weight:
614.88 (anhydrous basis)
MDL number:
MFCD00012116
UNSPSC Code:
12161900
PubChem Substance ID:
24892560
NACRES:
NA.21
Pricing and availability is not currently available.

biological source

bovine bile

Quality Level

400

grade

HPLC grade

description

zwitterionic

assay

≥98% (HPLC)

form

powder

mol wt

614.88 g/mol (anhydrous basis)

storage condition

dry at room temperature (tightly closed)

technique(s)

dialysis: suitable
electrophoresis: suitable

impurities

<0.04% DMF

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Show Differences

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Titanium rod, 500mm, diameter 1.5mm, annealed, 99.6+%

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Titanium rod, 500mm, diameter 3.0mm, annealed, 99.6+%

GF16772160

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Titanium rod, 500mm, diameter 5mm, annealed, 99.6+%

GF10374678

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Titanium rod, 1000mm, diameter 1.5mm, annealed, 99.6+%

GF34106363

Titanium

assay

99.6%

assay

99.60%

assay

≥99.6%

assay

99.6%

form

rod

form

rod

form

rod

form

rod

manufacturer/tradename

Goodfellow 038-382-82

manufacturer/tradename

Goodfellow 167-721-60

manufacturer/tradename

Goodfellow 103-746-78

manufacturer/tradename

Goodfellow 341-063-63

mp

1660 °C (lit.)

mp

1660 °C (lit.)

mp

1660 °C (lit.)

mp

1660 °C (lit.)

resistivity

42.0 μΩ-cm, 20°C

resistivity

42.0 μΩ-cm, 20°C

resistivity

42.0 μΩ-cm, 20°C

resistivity

42.0 μΩ-cm, 20°C

density

4.5 g/mL at 25 °C (lit.)

density

4.5 g/mL at 25 °C (lit.)

density

4.5 g/mL at 25 °C (lit.)

density

4.5 g/mL at 25 °C (lit.)

General description

CHAPS hydrate, a zwitterionic detergent derived from Cholic acid, stands out as an excellent tool in biochemical and cell biology research, particularly in the solubilization of membrane proteins. This versatile detergent, combining features of sulfobetaine-type and bile-acid detergents, exhibits a remarkable solubility rate of over 50% in aqueous solutions. It serves a pivotal role in stabilizing protein-DNA complexes, ensuring the retention of biochemical activity in solution.

Widely utilized as a buffer in various research applications, CHAPS hydrate acts as an amphiphilic surfactant, proving effective in solubilizing plasma membrane proteins and diluting assay components. Notably, its easy removal through dialysis or gel filtration adds to its appeal, making it a preferred choice for molecular mass determination by fluorometry. This feature underscores CHAPS hydrate′s adaptability in diverse experimental setups. However, caution is advised in assays involving polyvalent metals or metal ions sensitive to nonspecific chelating agents. Beyond its conventional use, CHAPS hydrate finds application as a polymer matrix in binding assays, effectively removing metal ions from protein solutions.

Application

0.2 w/v of CHAPS has been used to remove cerebral endothelium by brain perfusion.[1] 2% (w/v) CHAPS has been used as rehydration buffer along with Thiourea and Urea in protein extraction.[2] 10 mM CHAPS has been used along with 0.15 M NaCl, Tris–Cl, pH 7.6, EDTA as lysis buffer in Western Blot analysis.[3] CHAPS has been used as hybridization buffer in in-situ hybridization method of immature mammalian CNS.[4]

Features and Benefits

  • Suitable for Biochemical and Cell Biology research
  • Can be used in Dialysis and Electrophoresis
  • High purity product for research applications

Other Notes

For additional information on our range of Biochemicals, please complete this form.

comparable product

Product No.
Description
Pricing

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCJ8645
    SLCM1285
    SLCM1282
    SLCM1284
    SLCM1283

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    Febs Letters, 391(1-2), 199-202 (1996)
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