M0678

Middlebrook OADC Growth Supplement

Enrichment supplement recommended for isolation and cultivation of Mycobacteria, suitable for microbiology

• Synonym(s): Middlebrook Oleic Albumin Dextrose Catalase Growth Supplement, Oleic Albumin Dextrose Catalase
• UNSPSC Code: 41171614
• NACRES: NA.85

Properties

• Quality Level: 200
• sterility: sterile (Filtered and Aseptic Handled)
• form: liquid
• shelf life: limited shelf life, expiry date on the label
• packaging: vial of 50 mL
• application(s): clinical testing; environmental; food and beverages; life science and biopharma; veterinary; microbiology
• storage temp.: 2-8°C
• suitability: mycobacteria

Description

General description

Middlebrook OADC Growth Supplement is used with the Middlebrook Agar, that allows the isolation and cultivation of a wide variety of Mycobacteria. Middlebrook OADC Growth Supplement contains oleic acid, bovine albumin, sodium chloride, dextrose, and catalase. Oleic acid and other long chain fatty acids are essential for the metabolism of Mycobacteria. Some free fatty acids are toxic to Mycobacteria, but albumin binds to those fatty acids and prevents toxic action on Mycobacteria. Dextrose is the fermentable carbohydrate and an energy source whereas catalase destroys toxic peroxides that may be present in the medium.

Application

Middlebrook OADC growth supplement has been used as a supplement in 7H9 liquid medium to culture mycobacteria from clinical samples, environmental, food and beverages.

Components

Ingredients: (per vial)
Bovine Albumin Fraction V 2.50 g
Dextrose 1.00 g
Catalase 0.002 g
Oleic acid 0.025 g
Sodium chloride 0.425 g
Distilled water 50 mL

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves