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26745

Sigma-Aldrich

Cholesterol Esterase from porcine pancreas

lyophilized, powder, white, ~35 U/mg

Synonym(s):

bile salt-dependent lipase, bile salt-stimulated lipase, carboxyl ester lipase, nonspecific lipase, pancreatic lysophospholipase, Cholesterol Esterase from hog pancreas, Sterol-ester acylhydrolase

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About This Item

CAS Number:
EC Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.77
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biological source

Porcine pancreas

Quality Level

form

powder

quality

lyophilized

specific activity

~35 U/mg

mol wt

Mr ~440000

color

white

storage temp.

−20°C

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This Item
C376646058E0258
specific activity

~35 U/mg

specific activity

≥200 units/g protein

specific activity

≥50 U/mg

specific activity

≥4.0 units/mg protein (biuret)

biological source

Porcine pancreas

biological source

-

biological source

Porcine liver

biological source

Porcine pancreas

form

powder

form

lyophilized powder

form

lyophilized solid, powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

Mr ~440000

mol wt

84 kDa by SDS-PAGE

mol wt

Mr ~162000

mol wt

-

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

200

General description

Research area: Cell signaling

Pancreatic cholesterol esterase (CEase), also known as bile salt stimulated lipase, is a serine hydrolase belonging to the α/β hydrolase family of enzymes.[1] They are released from the exocrine pancreas. Serine 194, histidine 435 and aspartate 320 represent the catalytic triad of the enzyme.[1]

Application

Cholesterol Esterase from porcine pancreas has been used:
  • in an in vitro simulated digestion model to improve the hydrolysis of carotenoid esters in the intestinal phase[2]
  • to analyze the bioavailability of phytosterol and cholesterol in the aqueous micellar phase of an in vitro simulated digestion model[3]
  • as a standard in enzyme activity assay[4]

Biochem/physiol Actions

Cholesterol esterase (CEase), an enzyme having broad substrate specificity plays a key role in regulating the bile salt mediated hydrolysis of dietary cholesteryl esters. It also participates in the hydrolysis of triglycerides and phospholipids which are essential for normal cholesterol absorption.[1][5] Cholesterol esterase hydrolysis helps in the determination of total cholesterol levels in the serum and plasma. Additionally, cholesterol esterase (CEase) along with phospholipase A2 is involved in the hydrolysis of lecithin to lysolecithin, forming intestinal micelles that deliver [1] free cholesterol to enterocytes. Loss of enzyme function results in reduced intestinal absorption of dietary cholesteryl esters and prevents the formation of intestinal micelles, thereby failing to deliver cholesterol efficiently.[5] Circulating cholesterol esterases, accumulated in atherosclerotic lesions are involved in triggering the proliferation of smooth muscle cells.[1] Lastly, they are also selectively involved in the hydrolysis/condensation of carboxylic ester bonds.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol cholesterol per minute at pH 7.0 and 37°C (cholesterol acetate as substrate)

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Sales restrictions may apply.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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