32884

Giemsa Stain, Modified Solution

according to Giemsa

• Synonym(s): Azure eosin methylene blue, Giemsa solution
• CAS Number: 51811-82-6
• MDL number: MFCD00081642
• UNSPSC Code: 12171500
• PubChem Substance ID: 329754377
• NACRES: MA.02

Properties

• form: liquid
• Quality Level: 200
• quality: according to Giemsa
• technique(s): UV/Vis spectroscopy: suitable
• color: dark blue
• pH: 6.9
• density: 1.036 g/cm³ at 20 °C
• suitability: passes test for UV spectrum
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1
• InChI: 1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
• InChI key: NALREUIWICQLPS-UHFFFAOYSA-N

Description

General description

Modified Giemsa stain means when the pH of the Giemsa stain is changed from the usual 6.8 to 9.0.

Application

Modified Giemsa stain may be used for the identification of each human chromosome by differentials staining.

Other Notes

Giemsa stain, modified, 0.4%, w/v, in buffered methanol solution, pH 6.8. Also contains stabilizers.

Principle

When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

Safety Information

• pictograms: GHS02,GHS06,GHS08
• signalword: Danger
• hcodes: H225,H301 + H311 + H331,H370
• pcodes: P210 - P233 - P280 - P301 + P310 - P303 + P361 + P353 - P304 + P340 + P311
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
• target_organs: Eyes,Central nervous system
• Storage Class: 3 - Flammable liquids
• wgk_germany: WGK 2
• flash_point_f: 51.8 °F - closed cup
• flash_point_c: 11 °C - closed cup