35845

DCFHDA

BioReagent, suitable for fluorescence, ≥95% (HPLC)

• Synonym(s): 2′,7′-Dichlorofluorescein diacetate, Diacetyldichlorofluorescein
• Empirical Formula (Hill Notation): C₂₄H₁₄Cl₂O₇
• CAS Number: 2044-85-1
• Molecular Weight: 485.27
• Beilstein/REAXYS Number: 68488
• MDL number: MFCD00037501
• UNSPSC Code: 12352108
• PubChem Substance ID: 57649438
• NACRES: NA.32

Properties

• product line: BioReagent
• Quality Level: 100
• assay: ≥95% (HPLC)
• mp: 232-234 °C (lit.)
• solubility: DMSO: soluble
• fluorescence: λ_ex 504 nm; λ_em 524 nm in 0.1 M Tris pH 8.0 (esterase)
• suitability: suitable for fluorescence
• storage temp.: 2-8°C
• SMILES string: CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
• InChI: 1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3
• InChI key: VQVUBYASAICPFU-UHFFFAOYSA-N

Description

General description

2′,7′-Dichlorofluorescein diacetate, also known as DCFHDA or Dcfh-DA, is a sensitive cell-permeable redox probe. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.

Application

2′,7′-Dichlorofluorescein diacetate is predominantly used to detect reactive oxygen (ROS) intermediates and quantify the overall oxidative stress in cells.

Dcfh-DA is used in the quantitative assay to measure oxidative stress in nanoparticle-treated cells.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves