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51410

Sigma-Aldrich

Nε-(N-(+)-Biotinyl-6-aminohexanoyl)-Nα,Nα-bis(carboxymethyl)-L-lysine tripotassium salt

≥98.0% (TLC)

Synonym(s):

Biotin-X-NTA

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About This Item

Empirical Formula (Hill Notation):
C26H40K3N5O9S
CAS Number:
Molecular Weight:
715.98
UNSPSC Code:
12352209
NACRES:
NA.32

Pricing and availability is not currently available.

Quality Level

assay

≥98.0% (TLC)

form

solid

SMILES string

[K+].[K+].[K+].[O-]C(=O)CN(CC([O-])=O)[C@H](CCCCNC(=O)CCCCCNC(=O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@H]12)C([O-])=O

InChI

1S/C26H43N5O9S.3K/c32-20(28-13-7-5-8-18(25(38)39)31(14-22(34)35)15-23(36)37)10-2-1-6-12-27-21(33)11-4-3-9-19-24-17(16-41-19)29-26(40)30-24;;;/h17-19,24H,1-16H2,(H,27,33)(H,28,32)(H,34,35)(H,36,37)(H,38,39)(H2,29,30,40);;;/q;3*+1/p-3/t17-,18+,19-,24-;;;/m0

InChI key

MKLSOXPHWHEJPK-NERIWEOLSA-K

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This Item
444252444288444249
assay

≥95% (HPLC)

assay

≥95% (HPLC)

assay

≥95% (HPLC)

assay

≥95% (HPLC)

form

solid

form

solid

form

solid

form

solid

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

solubility

DMSO: 100 mg/mL

solubility

methanol: 1 mg/mL, DMSO: 100 mg/mL

solubility

methanol: 100 mg/mL, DMSO: 200 mg/mL

solubility

DMSO: 100 mg/mL, methanol: 25 mg/mL

Application

Nε-(N-(+)-Biotinyl-6-aminohexanoyl)-Nα,Nα-bis(carboxymethyl)-L-lysine (Biotin-X-NTA) is used as a hetero-bifunctional linker between histone(His)-tagged proteins and (strept)avidin conjugates.
Hetero-bifunctional linker for His-tagged proteins on one side and (strept)avidin conjugates[1][2][3]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Articles

Phospholipase A2 (PLA2) designates a class of enzymes that hydrolyze the sn-2 ester of glycerophospholipids to produce a fatty acid and a lysophospholipid. It has become clear that some of these enzymes liberate arachidonic acid in mammalian cells for the biosynthesis of eicosanoids, and thus there has been considerable interest in developing PLA2 inhibitors. Based on amino acid sequences, there are now more than 12 distinct groups of mammalian PLA2s, as well as many non-mammalian forms, all of which have been classified into 14 distinct groups with many subgroups.

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