55689

Alcohol Dehydrogenase equine

recombinant, expressed in E. coli, ≥0.5 U/mg

• Synonym(s): ADH
• CAS Number: 9031-72-5
• EC Number: 1.1.1.1 (BRENDA, IUBMB)
• EC Number: 232-870-4
• MDL number: MFCD00081305
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: equine
• Quality Level: 100
• recombinant: expressed in E. coli
• description: Isozyme E sequence
• form: lyophilized powder
• specific activity: ≥0.5 U/mg
• color: white; light yellow
• pH: 7
• solubility: water: 5 mg/mL
• application(s): life science and biopharma
• storage temp.: −20°C
• Gene Information: equine ... ADH1(111772995)

Description

General description

Research Area: Neuroscience
Alcohol dehydrogenase is a zinc metalloprotein that forms five classes of isoenzymes through the dimerization of eight different subunits.

Application

Alcohol Dehydrogenase equine has been used in in vitro alcohol dehydrogenase (Adh) assay.

Biochem/physiol Actions

Alcohol dehydrogenase catalyzes the oxidative conversion of alcohol into aldehyde. It has a homodimeric structure with a co-enzyme binding domain at the C-terminal and an N-terminal catalytic domain. The active site is located at the interdomain cleft. Binding of NAD+ in the active site causes conformational changes which create the binding site for the alcohol substrate.

Horse liver alcohol dehydrogenase (HL-ADH) is an enzyme with broad specificity, capable of catalyzing the reversible oxidation of a wide variety of primary and secondary alcohols to form their corresponding aldehydes and ketones. Moreover, alcohol dehydrogenase can oxidize ethanol while simultaneously reducing nicotinamide adenine dinucleotide (NAD+) to NADH. Previous studies have demonstrated that ADH and ALDH variants can influence alcohol dependence. Additionally, the ADH genotype has been linked to lacunar infarction and neuropsychiatric diseases.

Unit Definition

1 U corresponds to the amount of enzyme which reduces 1 μmol benzaldehyde per minute at pH 7.0 and 30 °C.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable