57620

Inulinase from Aspergillus niger

lyophilized, powder, brown-gray, ~25 U/mg

• CAS Number: 9025-67-6
• EC Number: 3.2.1.7 (BRENDA, IUBMB)
• EC Number: 232-802-3
• MDL number: MFCD00217463
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Aspergillus niger
• Quality Level: 100
• form: powder
• quality: lyophilized
• specific activity: ~25 U/mg
• color: brown-gray
• storage temp.: 2-8°C

Description

General description

Inulinases are classified as exoinulinases and endoinulinases.

Application

Inulinase from Aspergillus niger has been used as a glycoside hydrolase (GH) to study its potential to disperse bacteria (P. aeruginosa and S. aureus) from microbial biofilms using a polymicrobial well-plate model.

Biochem/physiol Actions

Inulinase hydrolyses inulin to produce oligosaccharides and liberate fructose. It also splits the terminal fructose units into sucrose and raffinose.

Inulinase is used in high-fructose syrup, which has therapeutic applications. Inulinase is reported to have high activity toward sucrose.

Unit Definition

1 U corresponds to the amount of enzyme which releases 1 μmol of reducing sugar (measured as fructose) per minute at pH 4.1 and 37°C from inulin

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)