84655
Schiff′s reagent for aldehydes
Synonym(s):
Fuchsin sulfurous acid
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About This Item
UNSPSC Code:
12352300
NACRES:
NA.21
form:
liquid
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This Item | 1.09033 | S5133 | F8129 |
|---|---|---|---|
| form liquid | form liquid | form liquid | form powder |
| Quality Level 100 | Quality Level 400 | Quality Level 100 | Quality Level 200 |
| grade for microscopy | grade - | grade - | grade - |
| application(s) food and beverages | application(s) clinical testing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
General description
Schiff′s reagent is used for the qualitative or quantitative assay of carbohydrates. Periodic acid-Schiff′s reagent (PAS) stain is broadly applicable in histology for visualizing glycogen, glycoproteins, mucins, and other polysaccharides in tissue and cells.
Application
Schiff′s reagent for aldehydes has been used:
- in microtiter PAS assay to quantify the amount of secreted mucin in Vibrio cholera infected water[1][2]
- to stain the colonies of Clostridium perfringens in PGY agar plates to identify plasmalogen-deficient mutants[3]
- in periodic acid-Schiff′s reagent assay to quantify carbohydrates in a microtiter plate format[4]
signalword
Danger
hcodes
Hazard Classifications
Carc. 1B - Met. Corr. 1
Storage Class
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Microtiter plate colorimetric assays are widely used for analysis of carbohydrates and glycoconjugates. However, mucins are often not easily detected, as they have low neutral sugar content. We have adapted and optimised the periodic acid-Schiff's reagent (PAS) staining for microtiter
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