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Sigma-Aldrich

Atto 532 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym(s):

Atto 532

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About This Item

MDL number:
MFCD05865401
UNSPSC Code:
12352108
NACRES:
NA.32
Pricing and availability is not currently available.
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product line

BioReagent

Quality Level

100

assay

≥90% (HPLC)
≥90% (degree of coupling)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

λ

in methanol: water (1:1) (with 0.1% perchloric acid)

UV absorption

λ: 532-538 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

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format

neat

format

neat

format

neat

format

neat

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

grade

analytical standard

grade

analytical standard

grade

analytical standard

grade

analytical standard

product line

PESTANAL®

product line

PESTANAL®

product line

PESTANAL®

product line

PESTANAL®

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

application(s)

agriculture
environmental

application(s)

agriculture
environmental

application(s)

agriculture
environmental

application(s)

agriculture
environmental

General description

Atto 532 NHS ester is a fluorescent dye related to the well-known laser dye, Rhodamine 6G. The fluorescence activity is excited efficiently at the 515-545nm range. A suitable excitation source for Atto 532 is the 532 nm output of the frequency-doubled Nd: YAG laser.

Application

Atto 532 NHS ester is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, and STED. In addition, the dye is used in flow cytometry (FACS) and fluorescence in-situ hybridization (FISH) methods.

Features and Benefits

Characteristic features of the Atto 532 NHS ester are:
  • Strong Absorption.
  • High Fluorescence quantum yield.
  • High Photostability.
  • Excellent water solubility.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCK3352
    BCCK1801
    BCCJ9358
    BCCJ0134
    BCCH8479

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