A8729
Akt1 active human
aqueous glycerol solution, recombinant, expressed in insect cells, protein apparent mol wt ~85 kDa, ≥90% (SDS-PAGE)
Synonym(s):
PKB α, Protein Kinase B-α
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About This Item
MDL number:
UNSPSC Code:
12352200
Application
Can be used in kinase activity assays, which measure the molar amount of phosphate incorporated into the Akt/SGK substrate peptide (RPRAATF) per minute per mg protein at 30 °C using a final concentration of 50 μM [32P]-ATP.
Biochem/physiol Actions
Akt1 is an isoform of multifunctional Akt kinase, which upon activation becomes involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, cell motility and a number of metabolic effects of insulin.
Other Notes
GST-tagged human recombinant full length Akt1
Unit Definition
One unit will incorporate 1 nanomole of phosphate into the AKT/SGK substrate peptide (RPRAATF) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm.
Physical form
Supplied as a solution at 0.1 mg/ml in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF and 25% glycerol.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Justin Kale et al.
EMBO reports, 19(9) (2018-07-11)
Akt is a pro-survival kinase frequently activated in human cancers and is associated with more aggressive tumors that resist therapy. Here, we connect Akt pathway activation to reduced sensitivity to chemotherapy via Akt phosphorylation of Bax at residue S184, one
Xue Bai et al.
Oncology reports, 33(6), 3085-3092 (2015-05-13)
Cervical cancer is the second most common women carcinoma worldwide and the fourth leading cause of cancer-associated mortality in women. Butein, a bioactive flavonoid isolated from numerous native plants, has been shown to induce apoptosis and inhibits migration and invasion
Shohei Shinozaki et al.
Science signaling, 7(351), ra106-ra106 (2014-11-13)
Inflammation increases the abundance of inducible nitric oxide synthase (iNOS), leading to enhanced production of nitric oxide (NO), which can modify proteins by S-nitrosylation. Enhanced NO production increases the activities of the transcription factors p53 and nuclear factor κB (NF-κB)
Pengfei Xu et al.
Molecular and cellular endocrinology, 406, 87-101 (2015-03-01)
White adipose tissue has now been recognized as an important endocrine organ secreting bioactive molecules termed adipocytokines. In obesity, anti-inflammatory adipocytokines like adiponectin are decreased while pro-inflammatory factors are over-produced. These changes contribute to the development of insulin resistance and
Tze-chen Hsieh et al.
PloS one, 9(6), e101070-e101070 (2014-06-27)
AKT plays an important role in the control of cell proliferation and survival. Aberrant activation of AKT frequently occurs in human cancers making it an attractive drug targets and leading to the synthesis of numerous AKT inhibitors as therapeutic candidates.
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