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A3316

Sigma-Aldrich

Anti-Human IgG (Fc Specific)−Agarose antibody produced in goat

affinity isolated antibody, PBS suspension

Synonym(s):

Goat anti-human IgG

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About This Item

MDL number:
MFCD02183598
UNSPSC Code:
12352203
NACRES:
NA.46
Pricing and availability is not currently available.

biological source

goat

Quality Level

200

conjugate

agarose conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

PBS suspension

species reactivity

human

should not react with

rat, mouse

technique(s)

Ouchterlony double diffusion: suitable

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Anti-Human IgG (Fc Specific)−Agarose antibody produced in goat affinity isolated antibody, PBS suspension

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Anti-Mouse IgG (Fab specific) antibody produced in goat 2.0 mg/mL, affinity isolated antibody

M4155

Anti-Mouse IgG (Fab specific) antibody produced in goat

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

goat

biological source

goat

biological source

goat

biological source

goat

conjugate

agarose conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

unconjugated

species reactivity

human

species reactivity

human

species reactivity

-

species reactivity

-

technique(s)

Ouchterlony double diffusion: suitable

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:55,000, dot blot: 1:40,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

technique(s)

Ouchterlony double diffusion: suitable, indirect ELISA: 1:15,000

form

PBS suspension

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

-

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
Goat Anti-Human IgG (Fc Specific)-Agarose antibody is specific for the Fc fragment of human IgG when tested against purified human IgA, IgG, IgM, Fc, and kappa and lambda light chains. No reactivity with mouse or rat IgG is seen by Ouchterlony Double Diffusion (ODD), prior to agarose bead coupling.

Immunogen

Fc fragment of human IgG.

Application

Anti-Human IgG (Fc Specific)-Agarose antibody produced in goat has also been used to purify galanin 1 (Gala1), 3Gal-substituted P-selectin glycoprotein ligand-1 (PSGL-1)/hIgG1 from supernatants of transfected COS cells.
Goat Anti-Human IgG (Fc Specific)-Agarose antibody is suitable for use in Ouchterlony double diffusion. The antibody has also been used for affinity purification assays.
Immunoprecipitation assays were performed using goat anti-human IgG (Fc specific) crosslinked to agarose beads. The beads were diluted in 1 ml of 5% FBS/DMEM for 2 hours at 4 degrees.

Other Notes

Antibody adsorbed with mouse and rat IgG

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
098M4862V
0000108212
059M4808V
21190120
21180653

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Rebecca J Brown et al.
The Journal of clinical endocrinology and metabolism, 102(6), 1789-1791 (2016-12-03)
Hyperinsulinemia can lead to pathologic ovarian growth and androgen production. A 29-year-old woman developed an autoantibody to the insulin receptor (type B insulin resistance), causing extreme insulin resistance and hyperinsulinemia. Testosterone levels were elevated to the adult male range. Treatment
J L Xu et al.
Immunity, 13(1), 37-45 (2000-08-10)
All rearranging antigen receptor genes have one or two highly diverse complementarity determining regions (CDRs) among the six that typically form the ligand binding surface. We report here that, in the case of antibodies, diversity at one of these regions
J Liu et al.
Transplantation, 63(11), 1673-1682 (1997-06-15)
The hyperacute rejection caused by preformed natural antibodies in the recipient species reacting with donor species endothelial antigens is one of the major obstacles preventing routine use of clinical xenotransplantation. Based on the known structure and biosynthetic pathway of the
S Sumitran et al.
Experimental neurology, 159(2), 347-361 (1999-10-03)
Transplantation of porcine embryonic brain cells, including dopaminergic neurons, from ventral mesencephalon (VM) is considered a potential treatment for patients with Parkinson's disease. In the present study, we characterized the distribution among VM cells of the major porcine endothelial xenoantigen
Jining Liu et al.
Xenotransplantation, 10(2), 149-163 (2003-02-18)
Hyperacute organ xenograft rejection can be prevented by removing anti-pig antibodies by extracorporeal absorption prior to transplantation. A novel recombinant absorber of anti-pig antibodies was developed by fusing the cDNA encoding the extracellular part of a mucin-type protein, P-selectin glycoprotein
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