A3316

Anti-Human IgG (Fc Specific)−Agarose antibody produced in goat

affinity isolated antibody, PBS suspension

• Synonym(s): Goat anti-human IgG
• MDL number: MFCD02183598
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: goat
• Quality Level: 200
• conjugate: agarose conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: PBS suspension
• species reactivity: human
• should not react with: rat, mouse
• technique(s): Ouchterlony double diffusion: suitable
• capacity: 2 mg/mL, resin binding capacity (human IgG)
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
Goat Anti-Human IgG (Fc Specific)-Agarose antibody is specific for the Fc fragment of human IgG when tested against purified human IgA, IgG, IgM, Fc, and kappa and lambda light chains. No reactivity with mouse or rat IgG is seen by Ouchterlony Double Diffusion (ODD), prior to agarose bead coupling.

Immunogen

Fc fragment of human IgG.

Application

Anti-Human IgG (Fc Specific)-Agarose antibody produced in goat has also been used to purify galanin 1 (Gala1), 3Gal-substituted P-selectin glycoprotein ligand-1 (PSGL-1)/hIgG1 from supernatants of transfected COS cells.

Goat Anti-Human IgG (Fc Specific)-Agarose antibody is suitable for use in Ouchterlony double diffusion. The antibody has also been used for affinity purification assays.

Immunoprecipitation assays were performed using goat anti-human IgG (Fc specific) crosslinked to agarose beads. The beads were diluted in 1 ml of 5% FBS/DMEM for 2 hours at 4 degrees.

Other Notes

Antibody adsorbed with mouse and rat IgG

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3