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A8438

Sigma-Aldrich

Anti-Rat IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Goat Anti-Rat IgG (whole molecule)–AP

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About This Item

MDL number:
MFCD00162809
UNSPSC Code:
12352203
NACRES:
NA.46
Pricing and availability is not currently available.

biological source

goat

Quality Level

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

technique(s)

direct ELISA: 1:30,000
immunohistochemistry (frozen sections): 1:50
western blot: 1:30,000

shipped in

wet ice

storage temp.

2-8°C

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Anti-Rat IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

A8438

Anti-Rat IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

A3687

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit affinity isolated antibody, buffered aqueous glycerol solution

A4187

Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

A3812

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

biological source

goat

biological source

goat

biological source

rabbit

biological source

goat

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

technique(s)

direct ELISA: 1:30,000, western blot: 1:30,000, immunohistochemistry (frozen sections): 1:50

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

General description

IgG, a monomer, is the predominant antibody circulating in blood which assist in phagocytic destruction of foreign microorganism. Anti-rat IgG (whole molecule)-alkaline phosphatase antibody (diluted 1: 30 000) can be used in rat MPO (myeloperoxidase) ELISA. It may also use as secondary antibody in immunodetection method. Anti-rat IgG (whole molecule)-alkaline phosphatase antibody reacts specifically with all rat immunoglobulins.

Immunogen

Purified rat IgG.

Application

Anti-rat IgG (whole molecule)–alkaline phosphatase antibody can be used in ELISA and western blot assays.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western blot was performed to analyze purified proteins over expressed in E.coli using alkaline-phosphatase-conjugated goat anti-rat IgG at a 1:1000 dilution. Western blot was developed using 5-bromo-4-chloro-3indolyl phosphate/nitro blue tetrazolium tablets (Sigma).

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 10 mM glycine, 50% glycerol and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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