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Key Documents

A9226

Sigma-Aldrich

Alkaline buffer solution

1.5 M, pH 10.3 (25 °C)

Synonym(s):

Basic pH buffer

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

form

liquid

Quality Level

concentration

1.5 M

pH

10.3 (25 °C)

storage temp.

2-8°C

General description

Alkaline buffer solution, 1.5 M, pH 10.3 at 25 °C is used in alkaline phosphatase reactions. It contains 2-amino-2-methylpropanol (AMP) and is used in conjunction with p-nitrophenyl phosphate (pNPP) enzyme substrate.

Application

Alkaline buffer solution has been used to measure the alkaline phosphatase (ALP) activity in the:
  • rat bone marrow stromal cells (BMSC)
  • osteoblast-like cells (SaoS-2, ATCC HTB 85)
  • mouse MC3T3-E1

Other Notes

Contains 2-amino-2-methylpropanol

pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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