B1059

Bcl-w (Minus C-Terminus) human

>95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

• MDL number: MFCD03846039
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• assay: >95% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• mol wt: 18 kDa by SDS-PAGE
• UniProt accession no.: Q92843
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... BCL2L2(599)

Description

General description

Lacks the mitochondrial targeting sequence but maintains the ability to neutralize pro-apoptotic Bcl-2 family members.

This recombinant Bcl-w lacks the mitochondrial targeting sequence but maintains the ability to neutralize pro-apoptotic Bcl-2 family members.

Biochem/physiol Actions

Anti-apoptotic member of the Bcl-2 family that prevents release of cytochrome c from the mitochondrial intermembrane space into the cytosol; promotes cell survival.

Like Bcl-2 and Bcl-x, the Bcl-w protein promotes cell survival, in contrast to other close homologues, Bax and Bak, which facilitate cell death. Bcl-w is an anti-apoptotic member of the Bcl-2 family that prevents release of cytochrome c from the mitochondrial intermembrane space into the cytosol. It is required for normal sperm maturation. Natural Bcl-w contains a C-terminal mitochondrial targeting sequence.

Physical form

Supplied as a 0.2 μm filtered solution in 25 mM HEPES, pH 7.5, 0.1 M KCl, and 10% glycerol.

Analysis Note

Measured by its ability to inhibit caspase-8-cleaved human BID-mediated release of cytochrome c from isolated mouse liver mitochondria using ELISA.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)