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B8041

Sigma-Aldrich

Biglycan from bovine articular cartilage

essentially salt-free, lyophilized powder

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.75

biological source

bovine articular cartilage

Quality Level

form

essentially salt-free, lyophilized powder

mol wt

200-350 kDa

packaging

glass bottle of 0.5 mg

technique(s)

cell culture | mammalian: suitable

solubility

water: soluble ≥2.00 mg/mL, clear, colorless

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

General description

proteoglycan consisting of a 45 kDa core protein and two chrondroitin/dermatan sulfate glycosaminoglycan chains

Biochem/physiol Actions

Biglycan interacts with collagen type I and II, fibronectin and TGF-ß. In a low ionic strength (less than 3 mM phosphate) buffer, Biglycan binds more strongly to collagen type I. At higher ionic strengths, Biglycan will instead increase the inhibition effect of TGF-ß on osteoclast proliferation at a concentration of 4-20 mg/mL. It also functions to prevent the attachment of CHO cells to fibronectin, with a 50% inhibition at 17-21 mg/mL. As an extracellular component, Biglycan binds TGF-β 1, facilitates bone mineralization and supports osteoblast differentiation thru BMP-4 binding.

Components

Biglycan is a 200-350 kDa proteoglycan consisting of a 45 kDa core protein and two chondroitin/dermatan sulfate glycosaminoglycan chains.

Caution

Store this product at -20°C. Stored as supplied, this powder shows little decomposition in 3 years when stored properly.

Preparation Note

This product is extracted from articular cartilage, chematographically purified, dialyzed against water, and 0.2 μm filtered prior to lyophilization. Once lyophilized, this powder is essentially salt-free. The product is soluble in water at 2 mg/mL.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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