B8041
Biglycan from bovine articular cartilage
essentially salt-free, lyophilized powder
Sign Into View Organizational & Contract Pricing
Select a Size
All Photos(1)
Select a Size
Change View
About This Item
Recommended Products
biological source
bovine articular cartilage
Quality Level
form
essentially salt-free, lyophilized powder
mol wt
200-350 kDa
packaging
glass bottle of 0.5 mg
technique(s)
cell culture | mammalian: suitable
solubility
water: soluble ≥2.00 mg/mL, clear, colorless
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
General description
proteoglycan consisting of a 45 kDa core protein and two chrondroitin/dermatan sulfate glycosaminoglycan chains
Biochem/physiol Actions
Biglycan interacts with collagen type I and II, fibronectin and TGF-ß. In a low ionic strength (less than 3 mM phosphate) buffer, Biglycan binds more strongly to collagen type I. At higher ionic strengths, Biglycan will instead increase the inhibition effect of TGF-ß on osteoclast proliferation at a concentration of 4-20 mg/mL. It also functions to prevent the attachment of CHO cells to fibronectin, with a 50% inhibition at 17-21 mg/mL. As an extracellular component, Biglycan binds TGF-β 1, facilitates bone mineralization and supports osteoblast differentiation thru BMP-4 binding.
Components
Biglycan is a 200-350 kDa proteoglycan consisting of a 45 kDa core protein and two chondroitin/dermatan sulfate glycosaminoglycan chains.
Caution
Store this product at -20°C. Stored as supplied, this powder shows little decomposition in 3 years when stored properly.
Preparation Note
This product is extracted from articular cartilage, chematographically purified, dialyzed against water, and 0.2 μm filtered prior to lyophilization. Once lyophilized, this powder is essentially salt-free. The product is soluble in water at 2 mg/mL.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
P J Roughley et al.
Matrix biology : journal of the International Society for Matrix Biology, 14(1), 51-59 (1994-01-01)
In chondrocytes isolated directly from human articular cartilage, without subsequent culture, biglycan mRNA levels decreased with the age of the donor, whereas those for decorin increased. In cultured chondrocytes in the presence of FCS, mRNA levels for biglycan remained similar
G Pogány et al.
Archives of biochemistry and biophysics, 313(1), 102-111 (1994-08-15)
Binding of proteoglycans to type I collagen in vitro was assessed using radiolabeled decorin, biglycan, and large proteoglycans and acid-extracted bovine tendon collagen. Decorin, biglycan, and large proteoglycans were all bound to collagen fibrils in phosphate-buffered saline (PBS) containing 3
E Schönherr et al.
The Journal of biological chemistry, 270(6), 2776-2783 (1995-02-10)
The small proteoglycan decorin is known to interact with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The structurally related proteoglycan biglycan has been proposed not to bind to fibrillar
Xiao-Dong Chen et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 18(9), 948-958 (2004-06-03)
Biglycan (bgn) is a small leucine-rich proteoglycan enriched in extracellular matrices of skeletal tissues. Bgn-deficient mice develop age-related osteopenia with a phenotype that resembles osteoporosis and premature arthritis. In the present study, we have examined the differentiation of bgn-deficient osteoblasts
Mauricio Moreno et al.
The EMBO journal, 24(7), 1397-1405 (2005-03-19)
The BMP4 signaling pathway plays key roles during early embryonic development and for maintenance of adult homeostasis. In the extracellular space, BMP4 activity is regulated by a group of interacting molecules including the BMP antagonist Chordin, the metalloproteinase Tolloid and
Articles
There are five identified glycosaminoglycan chains (see Figure 1): Hyaluronan is not sulfated, but the other glycosaminoglycan chains contain sulfate substituents at various positions of the chain.
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service