C0887

Chloroperoxidase from Caldariomyces fumago

buffered aqueous suspension, 1,000-2,000 units/mg protein (E1%/280)

• Synonym(s): Chloride Peroxidase, Chloride:hydrogen-peroxide oxidoreductase
• CAS Number: 9055-20-3
• EC Number: 1.11.1.10 (BRENDA, IUBMB)
• MDL number: MFCD00130774
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: fungus (Caldariomyces fumago)
• Quality Level: 200
• form: buffered aqueous suspension
• specific activity: 1,000-2,000 units/mg protein (E1%/280)
• mol wt: 42 kDa
• absorbance ratio: RZ ~1.0
• storage temp.: 2-8°C

Description

Application

A useful alternative to lactoperoxidase for ¹³¹I ion labeling studies, for bromination of proteins, and for ³⁶Cl labeling of macromolecules in long-term isolation procedures.

Biochem/physiol Actions

Chloroperoxidase (CPO) is a 42,000 Da extracellular heme glycoenzyme containing ferriprotoporphyrin IX as the prosthetic group. CPO is secreted from fungus and exhibits a broad spectrum of chemical reactivities. It is a peroxide-dependent chlorinating enzyme. It also catalyzes peroxidase-, catalase- and cytochrome P450-type reactions of dehydrogenation, H₂O₂ decomposition and oxygen insertion, respectively. The enzyme has magnetic and spectroscopic properties similar to that of cyctochrome P-450. CPO from the fungus Caldariomyces fumago has the capacity to chlorinate aromatic hydrocarbons, including polycyclic aromatic hydrocarbons (PAHs).

Unit Definition

One unit will catalyze the conversion of 1.0 μmole of monochlorodimedon to dichlorodimedon per min at pH 2.75 at 25 °C in the presence of potassium chloride and H₂O₂.

Physical form

Purified suspension in 0.1 M sodium phosphate solution, pH approx. 4.5

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)